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Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

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Updated: Jan 18, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

超分辨率兼容DNA标记技术揭示了在分化过程中染色体的移动性和组织变化.

Maruthi K Pabba¹, Miroslav Kuba^2,3, Tomáš Kraus²

¹Cell Biology and Epigenetics, Department of Biology, Technical University of Darmstadt, 64287, Darmstadt, Germany.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|September 9, 2025

概括

研究人员开发了一种新的探针,用于跟踪神经分化过程中活细胞中的染色质流动性. 这种方法表明,随着细胞的分化,染色素变得不那么可移动和可访问,从而提供了对细胞命运承诺的见解.

关键词:

苏联国家安全委员会子一个子染色体的移动性染色体的移动性在 dCSiRTPTP 中.人类iPSC的差异化

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Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

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相关实验视频

Last Updated: Jan 18, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

科学领域:

细胞生物学细胞生物学
神经科学是一个神经科学.
遗传学是一个遗传学.

背景情况:

染色质动力学对于细胞分化至关重要,但研究活细胞中的全球染色质流动性是具有挑战性的.
现有的用于观察分化过程中的染色质行为工具是有限的.

研究的目的:

开发和验证一种新的代谢标记探针,用于跟踪活细胞中的染色质动态.
研究神经分化过程中染色质流动性和组织的变化.

主要方法:

使用一种新型的罗达胺结合脱氧西提丁三酸盐 (dC^SiRTP) 通过合成输送器 (SNTT1) 传递的染色质的代谢标记.
相对对焦和STED超分辨率显微镜用于量化染色体域大小.
超分辨率显微镜与单个粒子跟踪分析染色质的移动性.
微球菌核酶消化试验,染色体紧缩和基因组修饰分析.

主要成果:

该dC^SiRTP探针已成功输入人体诱导多能干细胞 (iPSC) 和神经干细胞 (NSC).
在从iPSC转移到神经元的过程中,染色质的移动性显著下降.
神经元差异化与染色质可访问性降低和与异染色质相关的基因组修饰增加相关.

结论:

开发的探测器可以实时跟踪活细胞在分化过程中的染色质动态.
染色体经历结构变化,在神经发生过程中变得更紧,更难以接近.
这些发现为细胞命运承诺期间的染色质组织调节提供了新的见解.