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Updated: Jan 17, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
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4-Pi刺激拉曼散射用于无标签的超分辨率化学成像.

Jonathan I Kim1, Zachary Ellsworth1, Erin L Dunnington1

  • 1Department of Chemistry, University of Washington, Seattle, WA 98195, United States.

bioRxiv : the preprint server for biology
|September 15, 2025
PubMed
概括

这项研究引入了4Pi超高分辨率刺激拉曼散射 (SRS) 显微镜,提高了无标签化学成像的轴分辨率的七倍. 这一突破使得细胞结构的详细可视化无需分子标签.

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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