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相关概念视频

Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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pre-mRNA Processing02:01

pre-mRNA Processing

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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CAR-SPLASH识别出涉及动态合和替代拼接的新生mRNA前结构.

Hossein Shenasa1, Nova Fong1, Benjamin Erickson1

  • 1RNA Bioscience Initiative, Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045.

Proceedings of the National Academy of Sciences of the United States of America
|September 29, 2025
PubMed
概括

研究人员发现了RNA结构,称为"RNA动态开关",控制mRNA前拼接. 用反感性寡核酸破坏这些开关会改变替代拼接结果,揭示了转录和拼接之间的动态合机制.

关键词:
结构 RNA 结构 RNA 结构另一个替代拼接方法是拼接.动力合 动力合皮索拉伦交叉连接转录延长 延长 转录延长

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科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 基因规则 基因规则

背景情况:

  • 前信使RNA (前mRNA) 拼接与转录发生动态合,转录速度影响替代拼接 (AS) 结果.
  • 这种运动合背后的精确分子机制尚未完全理解.
  • 一个拟议的机制涉及新生的前mRNA的延长率依赖的替代折叠.

研究的目的:

  • 为了研究新生的前mRNA中的RNA结构,这些结构可能会调节动力合到拼接.
  • 识别和描述控制替代拼接的新型监管要素.
  • 探索针对这些结构的潜力,用反意义寡核酸 (ASO) 来进行拼接调制.

主要方法:

  • 修改松烯交联,结合和选择混合体 (SPLASH) 到染色质关联RNA (CAR) -SPLASH的测序.
  • 将CAR-SPLASH应用于具有野生类型和缓慢突变RNA聚合酶II的细胞.
  • 使用反感性寡核酸 (ASO) 破坏已识别的RNA复合体.

主要成果:

  • 在新生的前mRNA中识别了3000多个分子内RNA复合体,其中400多个位于结合点附近.
  • 通过ASO介导的特定双重点的破坏,称为"RNA动态开关",显著影响了替代拼接.
  • 这些对NISCH,GAK和MEGF8外型的AS的影响取决于转录延长率.

结论:

  • RNA动态开关通过调节新生的RNA结构折叠来调节转录和剪接之间的动态合.
  • 转录速度影响RNA折叠,这反过来影响了替代拼接结果.
  • 新生RNA结构代表了拼接修饰ASO的潜在目标.