Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Splicing01:32

RNA Splicing

60.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.3K
Alternative RNA Splicing02:18

Alternative RNA Splicing

24.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.7K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.8K
4.8K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.6K
6.6K
Leaky Scanning02:28

Leaky Scanning

5.6K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K
pre-mRNA Processing02:01

pre-mRNA Processing

57.1K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Flexible Teachers, Thriving Classrooms: A Unified Flexibility and Mindfulness (UFM) Model of Classroom Dynamics, Teaching Practices, and Teacher Burnout.

Behavioral sciences (Basel, Switzerland)·2026
Same author

Divergent RNA structures support accurate splicing of the SF3B1-sensitive <i>MAP3K7</i> intron.

bioRxiv : the preprint server for biology·2026
Same author

Management of Gynaecological Chronic Pelvic Pain in the Emergency Setting- A Scoping Review.

The Australian & New Zealand journal of obstetrics & gynaecology·2026
Same author

Assessing the Efficiency and Quality of Audio-Coding Versus Transcript Coding.

Nursing research·2026
Same author

Conservation of extended sequence and structure in the branchpoint-to-3' splice site region upstream of neural microexons.

bioRxiv : the preprint server for biology·2026
Same author

COVID-19 Vaccination Knowledge, Attitudes, Perception, and Practices Among Frontline Healthcare Workers in Tunisia, 2024.

Vaccines·2026

相关实验视频

Updated: Jan 15, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.9K

前体RNA在SF3B1突变敏感的神秘3'拼接部位的结构模式.

Austin Herbert1, Abigail Hatfield1, Alexandra Randazza1

  • 1Department of Genetics and Biochemistry, Center for Human Genetics, Clemson University, Clemson, South Carolina, USA.

RNA biology
|October 8, 2025
PubMed
概括

在血液疾病中常见的SF3B1 K700E突变改变了拼接部位的识别. 对于SF3B1敏感的加密站点具有较弱,灵活的序列,使其与耐SF3B1站点区别开来.

关键词:
3连接部位接部位结构 RNA 结构 RNA 结构在SF3B1中.隐秘的拼接地点.骨髓分裂性综合征是什么?拼接一个osomeome.

更多相关视频

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.2K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.4K

相关实验视频

Last Updated: Jan 15, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.9K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.2K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.4K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 在RNA分离过程中.

背景情况:

  • SF3B1对结合体的功能至关重要,特别是对3'结合点的选择.
  • SF3B1 K700E突变在骨髓质疏松综合征和其他血液疾病中普遍存在.
  • 突变型SF3B1利用了神秘的3'拼接部位,但它们的特异性质仍然不清楚.

研究的目的:

  • 为了识别和表征SF3B1敏感的神秘3'拼接位.
  • 确定序列和结构特征,区分SF3B1敏感和SF3B1耐药的拼接部位.
  • 了解SF3B1突变如何影响替代拼接.

主要方法:

  • 生物信息分析以确定SF3B1敏感和耐药的密码拼接部位.
  • 对聚皮里米丁通道和正规拼接部位强度的序列分析.
  • 化学探测用于评估拼接部位的RNA结构可访问性.
  • 对RNA结构灵活性进行比较分析.

主要成果:

  • 确定了192个对SF3B1敏感的和2800个对SF3B1抗性的神秘的3'拼接点.
  • 对于SF3B1敏感的部位具有延伸的多胺基通道和较弱的侧面正规拼接部位.
  • RNA结构分析显示了类似的可访问性模式,但SF3B1敏感部位的差异较小,这表明灵活性更大.

结论:

  • 对于SF3B1敏感的拼接接口具有独特的序列和结构特性.
  • 这些结位包含弱,差异化不良的拼接部位,容易受到突变SF3B1.1.的改变识别.
  • 这些发现提供了对血液疾病中SF3B1介导的拼接缺陷的分子机制的洞察.