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相关概念视频

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
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牙组织衍生的半机体干细胞的转录基因分析.

Sema S Hakki1, S Buket Bozkurt2, Zehragul Ergul3

  • 1Department of Periodontology, Selcuk University, Konya, Türkiye.

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概括

这项研究比较了来自不同牙的牙介质干细胞 (MSC) 中的基因表达. 牙周带MSCs通过抑制T细胞增殖显示了免疫调节的最高潜力.

关键词:
多国中心的MSCs.这就是 PENK mRNA.牙组织 牙组织牙周关节带 牙周关节带纸 纸 纸是一种转录组分析 转录组分析

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科学领域:

  • 牙科干细胞是指牙科的干细胞.
  • 免疫调节是一种免疫调节.
  • 基因表达分析 基因表达分析

背景情况:

  • 来自牙组织 (牙周带和牙肉) 的间酶体干细胞 (MSC) 被研究其治疗潜力.
  • 了解各种牙类型 (牙,前牙,叶叶) 的MSCs的差异性基因表达和免疫调节特性至关重要.

研究的目的:

  • 为了比较全基因组基因表达的牙周带 (PDL) 和肉质 (P) 中介质干细胞 (MSCs) 从第三牙,前牙和叶牙.
  • 根据差异表达基因,评估这些MSC的免疫调节特性.
  • 评估MSCs对T细胞增殖和亡的影响.

主要方法:

  • 从不同类型的牙中分离PDL和肉质MSC.
  • 使用人类表达混合化试验 (47,000个探针) 进行全基因组基因表达分析.
  • 与淋巴细胞一起培养MSC,以评估T细胞增殖 (WST-1检测) 和亡 (流细胞计).
  • 使用RT-PCR对特定基因表达 (PENK) 的验证.

主要成果:

  • 291个基因在不同牙类型和来源的MSC之间被差异表达 (≥2倍).
  • 观察到的显著差异是基法林 (PENK),表皮生长因子类蛋白6 (EGFL6) 和补充因子D (CFD) 的基因表达.
  • 与单独的T细胞相比,所有牙科MSC都抑制了T细胞的增殖 (p=0.001).
  • 前腹PDLMSCs表现出更高的T细胞增殖抑制和更高的PENK和IL-10mRNA表达.

结论:

  • 牙科MSC具有免疫调节能力,特别是抑制T细胞增殖.
  • 牙周带MSCs,特别是从前,显示出显著的免疫调节潜力,由于高PENK和IL-10表达.
  • 这些发现表明PDLMSCs是免疫相关疗法的有希望的候选者.