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Translocation of Proteins into the Mitochondria

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...

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Updated: Jan 15, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

使用TOMAHAQ对MHC-I的有针对性的分析

Marlene L Heberling¹, Sam Pollock², Qui T Phung¹

¹Proteomic and Genomic Technologies, Genentech, South San Francisco, CA, USA.

Methods in molecular biology (Clifton, N.J.)

|October 14, 2025

概括

我们开发了一种半自动化方法来丰富主要基因相容性复合物I类 (MHC-I) ,提高免疫治疗研究的吞吐量和灵敏度. 这种技术使MHC-I体的快速,可重现的分析成为可能,以更好地了解免疫调节.

关键词:

绝对量化 (TOMAHAQ) 是一个方法.精确的质量-质量.关联性净化净化方法癌症免疫学癌症免疫学高分辨率的高分辨率免疫类药物免疫类药物主要组织相容性复合物I类 (MHC-I)质谱测量质量谱测量多重复合的多重复合的新抗原是一种新抗原.半自动化半自动化半自动化由偏移引发的触发.

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Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

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相关实验视频

Last Updated: Jan 15, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

科学领域:

免疫学免疫学免疫学
蛋白质组学是指蛋白质组学.
质谱测量质量谱测量

背景情况:

主体组织相容性复合体 (MHC) 体组学对于理解免疫调节和开发免疫疗法至关重要.
目前用于隔离和量化MHC结合的方法具有挑战性,限制吞吐量,灵敏度和可重复性.
为了在各种细胞条件下快速表征MHC体,需要进行改进.

研究的目的:

描述一种强大,敏感和定量方法来丰富MHC-I结合.
为了使MHC-I从多种细胞系中实现高通量,半自动隔离.
引入TOMAHAQ (通过偏移触发,复合,精确质量,高分辨率和绝对量化) 用于针对MHC-I的向质谱.

主要方法:

半自动丰富MHC-I,使用可重复使用的抗体盒.
适用于具有挑战性的附着细胞系,如MC38.8.
使用TOMAHAQ,一种针对性质谱技术,用于多重和绝对量化.

主要成果:

在最短的实践时间内实现了MHC-I的可重复丰富,每天可进行多达96次同时丰富.
通过使用非向方法,对来自2.5亿个细胞的4000多个独特的MHC-I进行了高通量分析.
托马哈克提供了定量灵敏度,低至低的amol/μL水平,用于新表位特征.

结论:

开发的半自动MHC-I丰富方法显著提高了吞吐量,灵敏度和可重复性.
这种方法有助于详细描述MHC体和T细胞受体标的免疫疗法.
托马哈克与强大的分离相结合,为癌症研究中的定量新抗原分析提供了强大的工具.