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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

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Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
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相关实验视频

Updated: Jan 15, 2026

Eukaryotic Polyribosome Profile Analysis
09:16

Eukaryotic Polyribosome Profile Analysis

Published on: June 15, 2010

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为新型抗原发现进行核糖体分析.

Ana Xavier-Magalhães1, Susan Klaeger2

  • 1Genentech Inc., South San Francisco, CA, USA.

Methods in molecular biology (Clifton, N.J.)
|October 14, 2025
PubMed
概括
此摘要是机器生成的。

发现新的癌症抗原是免疫治疗的关键. 核糖体分析和质谱学识别了新目标,如新型开放阅读框架 (nuORFs),改善了癌症疫苗的开发.

关键词:
免疫皮组是一种免疫皮组.质谱测量质量谱测量没有NGS,没有NGS.新抗原是一种新抗原.新的或没有注释的开放阅读框架 (nuORFs).蛋白质基因组学是什么里博-seqq 的意思与瘤相关的抗原.没有注释的ORF.

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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
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相关实验视频

Last Updated: Jan 15, 2026

Eukaryotic Polyribosome Profile Analysis
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Eukaryotic Polyribosome Profile Analysis

Published on: June 15, 2010

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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

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科学领域:

  • 在瘤学瘤学.
  • 免疫学 免疫学 免疫学
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 传统的癌症抗原发现方法错过了新的目标,如拼接异型,基因融合和新的或未注释的开放阅读框架 (nuORF).
  • 这些新型抗原由于其潜在的丰富性和免疫性,为癌症免疫疗法提供了有前途的机会.

研究的目的:

  • 探索利用核糖体分析 (Ribo-seq) 和基于质谱 (MS) 的蛋白质组学,用于新型癌症抗原的发现.
  • 突出结合Ribo-seq与蛋白质基因组方法的潜力,以确定新的免疫治疗点.

主要方法:

  • 核糖体分析 (Ribo-seq) 用于评估和验证新型或未注释的开放阅读框架 (nuORF) 的翻译.
  • 基于质谱 (MS) 的蛋白质组学,以验证Ribo-seq.识别的蛋白质和的表达.
  • 蛋白质基因组方法整合Ribo-seq和MS数据,用于全面的抗原发现.

主要成果:

  • Ribo-seq有效地识别翻译的nuORFs,提供了丰富的潜在癌症抗原的来源.
  • 基于MS的蛋白质组学验证了这些新型翻译产物的表达,证实了它们作为抗原的存在.
  • 综合蛋白质基因组策略增强了新型癌症抗原的发现,而传统方法无法发现这些抗原.

结论:

  • 将Ribo-seq与基于MS的蛋白质组合相结合,是发现新型癌症抗原的有力策略.
  • 这种方法显著扩大了开发更有效的癌症免疫疗法的潜在目标目录.
  • 对这些新型抗原的进一步研究可能会导致个性化癌症治疗的突破.