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相关概念视频

MicroRNAs01:22

MicroRNAs

3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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相关实验视频

Updated: Jan 15, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

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没有种子配对的功能性microRNA准.

Matthew H Hall1,2,3, Peter Y Wang1,2,3, Thy M Pham1,2,3

  • 1Howard Hughes Medical Institute, Cambridge, MA 02142, USA.

Nucleic acids research
|October 16, 2025
PubMed
概括

微RNAs (miRNAs) 可以通过3'-only结合位抑制基因表达,挑战种子区域配对的必要性. 这些罕见的部位表现出功能抑制,影响阿尔戈诺特蛋白质复合体的动态.

科学领域:

  • 分子生物学分子生物学
  • 基因规则 基因规则
  • 在RNA生物学,RNA生物学.

背景情况:

  • 微RNAs (miRNAs) 结合阿尔戈诺特 (AGO) 蛋白质以引导mRNA向转录后抑制.
  • 规范性miRNA功能依赖于种子区域互补性 (核酸2-7) 进行mRNA结合和抑制.
  • 非正典结合点的作用,特别是那些缺乏种子互补性的结合点,仍然不太了解.

研究的目的:

  • 调查异常miRNA结合点的结合和抑制能力,这些异常miRNA结合点缺乏种子互补性,但在miRNA 3 egion中表现出广泛的配对.
  • 为了比较3个只有extprime的miRNA结合位点的生物物理特性和功能结果,与正规的种子匹配位点进行比较.
  • 为了确定这些非正规的3个extprime-only网站在内源miRNA向中的流行率和意义.

主要方法:

  • 使用3个只有extprime和种子匹配的结合位点对Argonaute-miRNA-mRNA相互作用的生物物理表征.
  • 测试以测量结合动力学 (结合和解离率) 和抑制效率.
  • 通过不同类型的结合部位诱导的AGO-miRNA复合体构造的分析.

主要成果:

  • 只有3个extprime结合点的结合亲和力和抑制功效与顶级正规种子匹配点相比较.

更多相关视频

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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相关实验视频

Last Updated: Jan 15, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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  • 通过最小的额外种子配对,可以增强仅由3个extprime站点的抑制.
  • 这些位点呈现较慢的结合/解离率,并与种子匹配位点相比诱导明显的AGO-miRNA复合体构造.

    • 结论:

    • 种子区域的互补性不是严格要求miRNA结合和抑制,也不是访问指导RNA的3 extprime区域.
    • 只有3个extprime的位点代表了一种罕见但功能性的miRNA向类,其比例与其他已知的罕见功能位点相似.
    • 了解非正规的miRNA结合扩展了已知的miRNAs转录后基因调节的机制.