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相关概念视频

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

569
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
569
In Vitro Drug Dissolution: Alternative Methods01:17

In Vitro Drug Dissolution: Alternative Methods

190
Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
190
In Vitro Drug Dissolution: Compendial Testing Models II01:09

In Vitro Drug Dissolution: Compendial Testing Models II

237
Various dissolution methods are utilized to assess a drug’s dissolution rate, including the flow-through cell, paddle-over-disk, cylinder, and reciprocating disk methods.The flow-through cell apparatus (USP (United States Pharmacopeia) method 4) comprises a reservoir for the dissolution medium and a pump that propels the medium through the cell containing the test sample. This method is crucial for assessing modified-release dosage forms with minimally soluble active ingredients,...
237

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相关实验视频

Updated: Jan 13, 2026

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform
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在体外皮肤模型作为皮肤药物开发和安全性评估的非动物方法.

Viviana Stephanie Costa Gagosian1, Raquel Coronel2, Bruna Caroline Buss3

  • 1Graduate Program in Bioscience and Biotechnology, Carlos Chagas Institute (ICC/FIOCRUZ-PR), Curitiba 81310-020, PR, Brazil.

Pharmaceutics
|October 29, 2025
PubMed
概括

先进的体外皮肤模型为化品和制药安全性评估提供了对动物试验的伦理和高效替代方案. 这些复杂的模型增强了皮肤学研究和产品开发.

关键词:
新方法方法论 (NAMs)重建的人类皮肤模型.规则 条例 条例 规章 规章 规章组织工程是组织工程.专题交付是当前的交付方式.毒理学 毒理学 毒理学通过皮肤传递的传递.

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Last Updated: Jan 13, 2026

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科学领域:

  • 皮肤病学和毒理学
  • 生物技术和生物医学工程 生物技术和生物医学工程

背景情况:

  • 在体外皮肤模型已经有了显著的进化,由需要道德,高效和可靠的替代品动物试验驱动.
  • 增加的监管要求和伦理考虑推动了复杂的人类皮肤模型的开发.

研究的目的:

  • 审查各种类型的体外皮肤模型,它们的应用,以及最近的进展.
  • 探索体外皮肤模型在研究和工业中的优点,局限性和未来前景.

主要方法:

  • 审查关于体外皮肤模型开发和应用的现有文献.
  • 分析模型制造中的生物打印和芯片器官等技术.
  • 检查用于一般和特定疾病应用的模型.

主要成果:

  • 在体外皮肤模型中,利用多种细胞类型来模仿生理皮肤环境,进行精确的产品相互作用研究.
  • 应用范围包括毒性测试,皮肤学产品评估,皮肤衰老研究和药物开发.
  • 生物打印和器官芯片技术正在彻底改变模型创建,尽管血管化和建筑方面的挑战仍然存在.

结论:

  • 在体外皮肤模型对于推进皮肤学研究和化品行业至关重要,减少对动物试验的依赖.
  • 在模型开发中的持续创新有望为皮肤研究提供更准确和更具预测性的工具.
  • 解决血管化和皮肤结构方面的挑战将进一步提高这些先进模型的实用性.