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Maryam Hojjat Jodaylami1,2, Dominic Falardeau2,3, Antoine Malescot2,3

  • 1Département de chimie, Institut Courtois, Quebec Center for Advanced Materials, Regroupement québécois sur les matériaux de pointe, Université de Montréal, C.P. 6128 Succ. Centre-ville, Montréal, Québec H3C 3J7, Canada.

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概括

这项研究引入了内表面增强拉曼散射 (SERS) 内镜,用于实时,体内蛋白质在大脑中的监测. 在SERS内镜成功检测到S100β蛋白释放与大脑活动相关的小鼠.

关键词:
在S100ββ中,它是S100ββ.一个内镜传感器.光遗传刺激是一种光遗传性刺激.表面增强的拉曼散射作用触觉刺激是一种触觉刺激.

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科学领域:

  • 神经科学是一个神经科学.
  • 生物医学工程 生物医学工程
  • 分析化学 分析化学

背景情况:

  • 表面增强的拉曼散射 (SERS) 内镜为在深部大脑区域的微侵入性,高分辨率的体内蛋白质检测提供了潜在的潜力.
  • S100β是一种指向大脑活动和损伤的天体细胞蛋白.

研究的目的:

  • 开发和验证内SERS内镜,用于体内监测不同大脑区域的S100β蛋白水平.
  • 为了将S100β蛋白释放与小鼠不同程度的脑活动相关联.

主要方法:

  • 使用光纤与金纳米粒子和抗S100β抗体功能化的SERS内镜的开发.
  • 在小鼠大脑中SERS内镜的体内植入.
  • 利用皮层部区域 (CMA) 的光遗传刺激来诱导节奏性部运动 (RJMs),并观察大脑干中的S100β变化.
  • 评估体感皮层中S100β水平,以应对触觉刺激.

主要成果:

  • 在视觉遗传学诱导的RJMs期间,SERS内镜检测到三角体主感官核 (NVsnpr) 中S100β度显著增加.
  • 观察到较低,但可检测的S100β释放与轻度CMA刺激没有唤起RJMs.
  • 在体感皮层中的S100β水平与触觉刺激后已知的激活配置相关.

结论:

  • 内SERS内镜是一种可行的工具,用于在清醒的动物体内的蛋白质监测.
  • 开发的SERS内镜可以检测与特定大脑活动相关的S100β蛋白水平的动态变化.
  • 这项技术对理解深层大脑结构中的神经过程和疾病状态具有前景.