Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Growth of Cartilage and Bone Tissue01:27

Growth of Cartilage and Bone Tissue

4.0K
Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
4.0K
Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

8.3K
Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
8.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Preliminary evaluation of full volume strain measurement in patellar cartilage following osteochondral allograft transplantation using magnetic resonance imaging.

Frontiers in bioengineering and biotechnology·2026
Same author

Modular, Vascularized Hypertrophic Cartilage Constructs for Bone Tissue Engineering Applications.

Tissue engineering. Part A·2025
Same author

Osteoporosis GWAS-implicated <i>DNM3</i> locus contextually regulates osteoblastic and chondrogenic fate of mesenchymal stem/progenitor cells through oscillating miR-199a-5p levels.

JBMR plus·2024
Same author

A synthetic, closed-looped gene circuit for the autonomous regulation of RUNX2 activity during chondrogenesis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2024
Same author

Injectable Methacrylated Gelatin Hydrogel for Safe Sodium Hypochlorite Delivery in Endodontics.

Gels (Basel, Switzerland)·2023
Same author

Ligand Composition and Coating Density Co-Modulate the Chondrocyte Function on Poly(glycerol-dodecanedioate).

Journal of functional biomaterials·2023

相关实验视频

Updated: Jan 12, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.5K

在MSC基软骨形成期间长期抑制RUNX2会增加软骨矩阵积累.

Ruxin Yang1, Tiana J Wong1, Rhima M Coleman1,2,3

  • 1Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.

GEN biotechnology
|November 3, 2025
PubMed
概括

这项研究表明,抑制 RUNX2 基因表达在介质干细胞衍生型冠状细胞中,可以增强软骨基质的产生和机械强度. 这种RUNX2抑制为软骨组织工程和修复提供了一个有希望的策略.

更多相关视频

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
06:05

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

1.6K
Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair
04:48

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair

Published on: March 1, 2024

2.4K

相关实验视频

Last Updated: Jan 12, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.5K
Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
06:05

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

1.6K
Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair
04:48

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair

Published on: March 1, 2024

2.4K

科学领域:

  • 生物医学工程 生物医学工程
  • 再生医学是一种再生医学.
  • 分子生物学分子生物学

背景情况:

  • 软骨缺陷带来了重大的临床挑战.
  • 目前的组织工程策略需要加强,以获得长期的疗效.
  • RUNX2是冠状体发生的关键转录因子.

研究的目的:

  • 评估自主RUNX2抑制对软骨矩阵积累的长期影响.
  • 评估RUNX2抑制对工程软骨压缩机制的影响.
  • 调查RUNX2抑制基因电路在MSC衍生型冠状细胞 (MdChs) 中的疗效.

主要方法:

  • 人类介质干细胞 (MSCs) 分化为冠状细胞.
  • 使用向RUNX2 (shRUNX2) 的lentiviral短毛RNA (shRNA) 进行了基因修饰.
  • 实施和评估了shRUNX2的两个表达水平 (低和高).

主要成果:

  • 低和高的shRUNX2都有效地抑制了RUNX2的表达.
  • 观察到软骨矩阵积累的显著改善,包括II型原蛋白和亚格拉干.
  • 与对照组相比,高shRUNX2显示了硫酸糖氨基甘油和总体矩阵积累的优越增强.

结论:

  • 自主RUNX2抑制有效地增强了长期的软骨矩阵积累.
  • 抑制RUNX2可以改善工程软骨组织的机械性能.
  • 这种方法为推进软骨组织工程和修复提供了一个有希望的策略.