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相关概念视频

Pleiotropy01:33

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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相关实验视频

Updated: Jun 28, 2026

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
10:25

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

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在人类大脑小脑发育过程中,大脑小脑器官模型细胞类型特定的FOXP2表达.

Elizabeth J Apsley1,2, Joey Riepsaame3, Yin Chun Cheng1,2

  • 1Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford OX3 9DU, UK.

Disease models & mechanisms
|November 14, 2025
PubMed
概括

这项研究使用有机体和CRISPR基因编辑来模拟人类小脑早期发育. 研究人员在关键发育中的小脑细胞中确定了叉头盒蛋白P2 (FOXP2) 表达,揭示了它们对神经发育障碍的脆弱性.

关键词:
小脑核中的核.大脑小部分的大脑小部分叉头盒子 叉头盒子神经发育障碍 神经发育障碍普尔金尼细胞是什么?这就是 iPSC 的意义.

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Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining
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Modeling Human Cerebellar Development In Vitro in 2D Structure
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Modeling Human Cerebellar Development In Vitro in 2D Structure
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Modeling Human Cerebellar Development In Vitro in 2D Structure

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科学领域:

  • 神经科学是一个神经科学.
  • 发展生物学 发展生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 人类小脑发育是复杂的,动物研究模型很差.
  • 大脑小管器官为研究人类大脑发育和疾病提供了一个有前途的模型.
  • 叉头盒蛋白P2 (FOXP2) 在小脑早期发育中的作用在很大程度上是未知的.

研究的目的:

  • 使用有机体模型研究FOXP2在人类小脑早期发育中的作用.
  • 描述FOXP2表达细胞,并确定它们在发育中的小脑中的下游目标.
  • 了解FOXP2表达对神经发育障碍的影响.

主要方法:

  • 基于CRISPR的基因编辑被用于人类诱导的多能干细胞衍生的小脑器官.
  • 一个光FOXP2记者线被生成来跟踪FOXP2表达细胞.
  • 进行了转录基因分析和与现有数据集的交叉引用.

主要成果:

  • 在早期人类的普金尼细胞和小脑核神经元中发现了FOXP2的表达.
  • 确定了FOXP2在发展中小脑中的潜在下游点.
  • 这项研究强调了这些FOXP2表达细胞群对神经发育障碍的脆弱性.

结论:

  • 在人类小脑中,FOXP2在特定神经元群体的发展中起着重要作用.
  • 大脑小管器官是研究人类神经发育和相关疾病的宝贵工具.
  • 了解FOXP2的作用,可以了解影响言语和语言的神经发育状况的病因.