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相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

24.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Alternative RNA Splicing02:18

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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Chromatin Structure and RNA Splicing02:41

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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

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Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
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相关实验视频

Updated: Jan 6, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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circASbase:circRNAs中替代分离事件的综合数据库

Lingxiao Zou1, Jian Zhao1, Haojie Li2,3,4

  • 1Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China.

Genomics, proteomics & bioinformatics
|December 2, 2025
PubMed
概括
此摘要是机器生成的。

一个新的数据库,circASbase,对13种物种的45万多个循环RNA (circRNA) 替代拼接事件进行了目录. 该资源揭示了circRNAs中独特的拼接模式,影响了它们的功能和调节.

关键词:
另一个替代拼接.这是循环RNARNA.编码潜在的编码.数据库数据库数据库是一个数据库.全长度的异构形.

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 替代拼接产生各种RNA异型,包括循环RNA (circRNAs).
  • 缺乏专门的数据库阻碍了研究circRNA替代拼接事件.
  • 了解circRNA拼接对于它们的功能多样性至关重要.

研究的目的:

  • 开发circASbase,这是一个针对circRNA替代拼接事件的全面数据库.
  • 为研究circRNA拼接调节提供丰富的注释.
  • 促进研究circRNA替代拼接的功能作用.

主要方法:

  • 在884,047个全长circRNA中编目了452,129个替代拼接事件.
  • 分析了来自13个物种的581个样本的数据.
  • 开发用户友好的界面和基于Web的可视化工具.

主要成果:

  • 在circRNAs和线性转录之间的替代拼接中发现了实质性的差异.
  • 证明circRNA剪接事件会影响IRES站点,m6A站点,ORF和miRNA目标.
  • 展示了独特的监管格局和circRNA拼接的功能影响.

结论:

  • circASbase解决了对circRNA拼接数据库的需求.
  • 该数据库促进了对circRNA生物学和调控机制的理解.
  • circASbase是探索 circRNA 替代拼接和功能的宝贵资源.