Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Chromosome Replication02:31

Chromosome Replication

10.4K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.4K
DNA Replication02:40

DNA Replication

58.2K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
58.2K
The Replisome03:01

The Replisome

37.9K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
37.9K
The Replisome03:01

The Replisome

9.6K
9.6K
Next-generation Sequencing03:00

Next-generation Sequencing

97.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.6K
Chromosome Structure02:40

Chromosome Structure

25.8K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
25.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Rocker-Switch Mechanism of Choline Transmembrane Transport in FLVCR2: Dynamic Latch Residues and Cooperative Regulation of Transmembrane Helices.

JACS Au·2025
Same author

OriV-Finder: a comprehensive web server for bacterial plasmid replication origin analysis.

Nucleic acids research·2025
Same author

Dynamic Mechanism of Norepinephrine Reuptake and Antidepressants Blockade Regulated by Membrane Potential.

Journal of chemical theory and computation·2025
Same author

Nmix: a hybrid deep learning model for precise prediction of 2'-O-methylation sites based on multi-feature fusion and ensemble learning.

Briefings in bioinformatics·2024
Same author

DeOri 10.0: An Updated Database of Experimentally Identified Eukaryotic Replication Origins.

Genomics, proteomics & bioinformatics·2024
Same author

Impacts of different pancreatic resection ranges on endocrine function in <i>Suncus murinus</i>.

World journal of gastrointestinal surgery·2024

相关实验视频

Updated: Jan 8, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.8K

基于ResNet的序列模式的多类识别,用于基因组复制的时间分析.

Zhen-Ning Yin1, Yu-Hao Zeng1, Feng Gao2,3,4

  • 1Department of Physics, School of Science, Tianjin University, Tianjin, 300072, China.

Interdisciplinary sciences, computational life sciences
|December 12, 2025
PubMed
概括
此摘要是机器生成的。

本研究介绍了RT-Predictor,这是一种深度学习模型,只使用DNA序列模式准确预测DNA复制时间 (RT) 域. 该框架增强了对基因组稳定性及其与癌症等疾病的联系的理解.

关键词:
深度学习是一种深度学习.复制的起源 复制的起源复制时间复制时间.

更多相关视频

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.2K
Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
10:17

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

8.3K

相关实验视频

Last Updated: Jan 8, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.8K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.2K
Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
10:17

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

8.3K

科学领域:

  • 基因组学就是基因组学.
  • 计算生物学 计算生物学
  • 分子生物学分子生物学

背景情况:

  • 精确调节DNA复制时间 (RT) 对基因组稳定性至关重要.
  • 识别影响RT的序列模式是具有挑战性的,具有有限的计算工具来准确预测.
  • 了解RT动态对于破译基因组不稳定性和包括癌症在内的疾病机制至关重要.

研究的目的:

  • 开发基于深度学习的框架RT-Predictor,用于使用序列模式准确预测DNA复制时间域.
  • 将人类基因组序列分为四个不同的复制时间域:早期复制域 (ERD),下移转区 (DTZ),晚期复制域 (LRD) 和上移转区 (UTZ).
  • 研究特定序列模式,复制起源 (ORIs) 和与DNA损伤修复机制的潜在联系之间的关系.

主要方法:

  • 使用深度学习框架,特别是残余网络 (ResNet),用于序列模式分类.
  • 从DNA序列中提取了384个特征,包括基于位置和频率的特征,以定义复制动态.
  • 进行全基因组RT预测,分析复制时间域的分布和特征.

主要成果:

  • 通过仅使用DNA序列模式,RT-Predictor实现了74.58%的准确性,0.6612MCC,0.7458F1得分和0.7457回忆.
  • 该模型成功地解决了人类基因组中复杂的DNA复制时间模式.
  • 全基因组分析表明,复制起源主要在早期的S阶段启动复制,这表明基于序列模式的调节.

结论:

  • 深度学习有效地解码了DNA复制时间中的序列模式的监管意义.
  • RT-Predictor为分析复制动态和理解基因组稳定性提供了一个强大的工具.
  • 这些发现为基因组稳定的分子基础及其在疾病,特别是癌症中的破坏提供了关键的见解.