Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

10.0K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
10.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Nuclear speckle dynamics are controlled by polyphosphate inhibition of CLK proteins.

Nucleic acids research·2026
Same author

Targeting notch signaling to restore neural development and behavior in mouse models of ASD.

Nature communications·2026
Same author

Long-term effectiveness and safety of satralizumab for neuromyelitis optica spectrum disorder in a real-world clinical setting in Japan: A 2.5-year final analysis of a multicenter medical chart review (The SAkuraBeyond Study).

Multiple sclerosis (Houndmills, Basingstoke, England)·2026
Same author

Phytic acid (InsP<sub>6</sub>) activates HDAC3 epigenetic axis to maintain intestinal barrier function.

Nature communications·2026
Same author

Phosphorus-specific, liquid chromatography inductively coupled plasma mass spectrometry for analysis of inositol phosphate and inositol pyrophosphate metabolism.

The Biochemical journal·2025
Same author

Oxidation Reactions Promoted or Catalyzed by Organotellurium Compounds.

ACS omega·2025

相关实验视频

Updated: Jan 8, 2026

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

2.7K

分析方法和工具用于研究内醇酸盐.

Masatoshi Ito1,2, Shinichi Koguchi3, Adolfo Saiardi4

  • 1Department of Neurology, Tokai University School of Medicine, Isehara, Japan.

FEBS letters
|December 16, 2025
PubMed
概括

在生物样本中检测异醇酸盐 (InsPs) 是一个挑战. 最近分析化学的进步,包括质谱和色谱学,改善了InsP的检测和定量用于生物研究.

关键词:
毛细管电泳质谱学质谱学伊诺西酸的含量是多少伊诺西酸铁酸盐 是一种同位素标签的标签液态染色学 质谱学 质谱学质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量胺酸的化学成分

更多相关视频

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

7.5K
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

8.9K

相关实验视频

Last Updated: Jan 8, 2026

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

2.7K
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

7.5K
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

8.9K

科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 分子生物学分子生物学

背景情况:

  • 异醇酸盐 (InsPs) 起着至关重要的生物作用,但它们的全部意义尚未被理解.
  • 在复杂的生物样本中检测InsP是InsP研究的一个主要障碍.

研究的目的:

  • 审查因诺醇酸盐研究的历史和最新方法.
  • 为InsP研究突出分析技术和合成化学的进步.

主要方法:

  • 分析化学技术的审查:NMR光谱学,质谱学 (MS),液态染色学 (LC) 和毛细管电泳 (CE).
  • 讨论合成化学方法来创建InsPs及其类型.
  • 专注于MS与LC和CE相结合,用于敏感的InsP检测.

主要成果:

  • 分析化学的进步使得InsPs的敏感和选择性差异化成为可能.
  • 合成方法为绝对丰度量化和功能研究提供了工具.
  • 最近的协议将LC-MS和CE-MS结合起来,以改进InsP分析.

结论:

  • 分析化学专业知识越来越重要,因诺酸盐研究.
  • 新的方法提高了研究InsP生物和医学意义的能力.
  • 使用先进分析和合成的综合方法是未来InsP发现的关键.