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相关概念视频

RNA Structure01:19

RNA Structure

6.9K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
6.9K
RNA Structure01:23

RNA Structure

78.7K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.7K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.6K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.6K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.3K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.3K
RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K
RNA Splicing01:32

RNA Splicing

60.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.2K

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相关实验视频

Updated: Jan 8, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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关于现有的RNA片段结构的完整性

Xu Hong1, Jian Zhan1,2, Yaoqi Zhou1

  • 1Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen 518055, China.

Genomics, proteomics & bioinformatics
|December 19, 2025
PubMed
概括

由于其结构空间不完整,RNA结构预测落后于蛋白质. 需要新的实验方法来捕获更多的RNA结构,重点是稳定的糖环平台.

科学领域:

  • 结构生物学是结构生物学.
  • 计算生物学是一种计算生物学.
  • 生物物理学的生物物理.

背景情况:

  • 像AlphaFold 2这样的深度学习方法已经彻底改变了蛋白质结构的预测.
  • 蛋白质的结构空间在很大程度上是完整的,这有助于预测的准确性.
  • 由于结构数据不完整,RNA结构预测面临挑战.

研究的目的:

  • 评估RNA结构空间在各种核酸水平 (二,三,四,五) 的完整性.
  • 为了比较RNA结构空间与蛋白质结构空间的完整性.
  • 在RNA中识别稳定的结构动图,以便在预测模型中潜在使用.

主要方法:

  • 在二,三,四,五核酸水平上分析RNA的非冗余结构碎片.
  • 检查RNA片段中的结构多样性.
  • 在RNA结构中确定稳定的参考框架.

主要成果:

  • 在四核和五核酸水平上,非冗余RNA结构碎片的数量呈指数级增长.
  • 与蛋白质结构空间相比,RNA结构空间远远不完整.
  • 一个特定的参考框架 (O4',C1',C2') 显示最小的结构多样性,表明稳定性.
关键词:
碎片的RNARNA碎片的RNA碎片模拟RNA的RNA模型在RNA伪扭曲角度.一个RNA参考框架的RNA参考框架.结构 RNA 结构 RNA 结构

更多相关视频

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
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Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

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An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

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相关实验视频

Last Updated: Jan 8, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

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Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

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An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

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结论:

  • 需要大量的努力来提高RNA结构的实验性确定,以扩大观察到的结构空间.
  • 改进的方法对于提高RNA结构预测准确度至关重要.
  • 确定了稳定的糖环参考框架,可以作为RNA结构建模的基础元素.