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Updated: Jan 8, 2026

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL
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半合成多色探头用于生物发光成像.

Suong Tran1, Kat Usavage1, Lila P Halbers2

  • 1Department of Chemistry, Dickinson College, Carlisle, 17013-2896 Pennsylvania, United States.

ACS omega
|December 22, 2025
PubMed
概括

研究人员开发了一种新的生物发光成像方法,使用NanoBiT,一个分裂的光酶,以克服NanoLuc.

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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