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Development of Blood Vessels

Development of Blood Vessels

The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...

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Updated: Jul 10, 2026

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Published on: April 29, 2007

珠子:为3D纤维素血管生成试验提供交互式半自动化工作流程,使共同培养和方向性分析成为可能.

Cailin R Gonyea¹, Brielle Hayward-Piatkovskyi², Jason P Gleghorn^1,2

¹Department of Biomedical Engineering, University of Delaware, Newark, DE, USA.

bioRxiv : the preprint server for biology

|December 25, 2025

概括

一个新的半自动图像分析工具,基于珠子的内皮血管生成数据套件 (BEADS),在3D纤维素血管生成试验中量化了内皮芽生殖. BEADS显著减少了分析时间,同时允许详细的空间和共同文化评估.

关键词:

3D纤维素珠测定试验血管新生的产生.内皮共培养是一种内皮共培养.半自动化分析方法芽的方向性芽的方向性微血管成像技术的成像

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Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Published on: February 16, 2018

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Published on: September 18, 2019

相关实验视频

Last Updated: Jul 10, 2026

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Published on: April 29, 2007

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Published on: February 16, 2018

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Published on: September 18, 2019

科学领域:

* 生物医学工程 * 生物医学工程
* 细胞生物学 * 细胞生物学
* * 量化成像技术的使用

背景情况:

* 3D纤维素珠血管生成试验对于在体外研究内皮发芽至关重要.
*现有的分析方法耗时,难以处理复杂的成像,阻碍了对共同文化和空间相互作用的定量分析.
*需要标准化,高效的工具来分析复杂的实验设置中的血管生成.

研究的目的:

* 开发一个半自动化,用户交互式的图像分析管道,名为基于珠子的内皮血管生成数据套件 (BEADS).
*在3D纤维素血管生成试验中提供发芽,迁移和空间定向的标准化,定量,以珠子为中心的指标.
* 为了实现先进的分析,包括共同种植的指定,最近邻居配对和芽的方向性.

主要方法:

* 集成的自动珠子检测与手动校正精度.
* 在多通道图像z-stack中使用芽和迁移细胞的指导注释.
* 纳入了用于共同文化识别,近邻分析和循环统计数据的新功能.

主要成果:

*与手动追踪相比,BEADS可减少手动分析时间约7倍.
* 苗长度的准确性与手动地面准确性相比,得到了保留.
* 在与共同培养的人类肺微血管内皮细胞 (HPMECs) 的试验中表现出性能.

结论:

*BEADS提供了一个标准化,可扩展的微血管图像分析平台.
* 该工具支持共同培养实验,空间内皮细胞相互作用指标和迁移细胞量化.
* 这种半自动化工作流程通过将计算精度与生物见解相结合,增强了定量微血管研究.