相关实验视频

Updated: Jan 7, 2026

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

通过光板成像和2D空间多重复合进行3D引导切割和深层细胞表型的协议

Kevin Bigott¹, Victoria H Schoppel¹, Manuel Martinez-Osuna¹

¹Miltenyi Biotec B.V. & Co. KG, 51429 Bergisch Gladbach, Germany.

|December 28, 2025

概括

本研究介绍了一种高分辨率分析3D组织中的生物分子的方法. 它将光片成像与深层细胞表型化相结合,用于详细的空间分析.

科学领域:

生物成像成像技术
组织分析分析组织分析.
细胞表型的形成

背景情况:

了解生物过程需要对细胞及其组织内的微环境进行空间分析.
在亚细胞分辨率的大型3D组织样本中分析广泛的生物分子是理想的,但具有挑战性.

研究的目的:

提出一项用于从清除的组织中识别和提取目标部分的协议.
为了使生物分子在3D组织样本中的详细空间分析.

主要方法:

从清除的组织中进行切片识别和提取的协议.
结合3D光片成像的组合.
使用多循环二维显微镜进行3D引导深层细胞表型化.

主要成果:

成功识别和提取目标组织切片.
整合3D成像与先进的细胞表型化.
组织内的生物分子的高分辨率空间分析.

结论:

本协议为3D组织中生物分子的详细空间分析提供了便利.
这种方法增强了对细胞微环境和生物过程的理解.
结合先进的成像和表型,用于全面的组织分析.

关键词:

癌症癌症癌症癌症显微镜的使用方法神经科学是一个神经科学.

更多相关视频

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Published on: May 11, 2018

相关概念视频

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...