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相关概念视频

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Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
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Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
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相关实验视频

Updated: Jan 7, 2026

The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics
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内囊泡作为传染中间体

Nourhan A M Mahmoud1,2, Hadeer K S Abdelrahman1,2, Benedita K L Feron1,3

  • 1The Exogenix Laboratory, School of Science, University of Greenwich, Central Avenue, Chatham Maritime, Kent ME4 4TB, UK.

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PubMed
概括

减弱的炭毒素 (aATx) 通过内囊泡 (ILV) 促进了通过小干扰RNA (siRNA) 的增强细胞质传递,改善了基因沉默. 抑制ILVs显著减少aATx介导的siRNA转位和基因淘汰.

关键词:
炭病毒素是一种毒素.药物输送是药物输送的过程.细胞内症是指内细胞内症.内囊中的内囊siRNA 是一个RNA.

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科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物技术是生物技术.

背景情况:

  • 基于蛋白质架构的转染系统被探索,以管理肝分布和毒性.
  • 减弱的炭病毒素 (aATx) 作为一种新型传染系统的支架,利用内囊泡 (ILV) 进行细胞溶液输送.
  • 附在LFn-PKR上的小干扰RNA (siRNA) 并不是aATx转位酶 (保护性抗原 (PA)) 的预测基质.

研究的目的:

  • 量化aATx传递的siRNA的细胞内定位,并将其与基因沉默活性相关联.
  • 调查ILVs在siRNA的ATx介导细胞突变中的作用.
  • 与传统的转染方法相比,评估基于aATx的siRNA输送的有效性.

主要方法:

  • 放射性 (32P标记) siRNA被用于跟踪与aATx在MCF-7细胞中转移后的细胞内分布.
  • 基因沉默的有效性是通过西方分析来准STAT3基因表达来衡量的.
  • 使用高糖或小麦胚芽聚合素 (WGA) 抑制ILV形成,研究其对siRNA转位的影响.

主要成果:

  • aATx介导的转染导致细胞关联siRNA在细胞质中的77%±2.5%,与Lipofectamine的45%±3.2%相比.
  • aATx的输送导致了STAT3表达的显著减少 (64.04% ± 14.17%).
  • 通过WGA (75.23% ± 0.06%) 或砂糖 (74.58% ± 7.76%) 抑制ILV形成显著减少了STAT3基因敲击.

结论:

  • 保护性抗原 (PA) 孔插入内体膜不会损害膜完整性,防止囊泡破裂.
  • 内囊泡 (ILV) 在aATx系统的转位酶活性中发挥着关键作用.
  • aATx系统证明了siRNA的有效细胞溶液传递,导致有效的基因沉默,ILVs对于这一过程至关重要.