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相关概念视频

Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Epigenetic Regulation01:37

Epigenetic Regulation

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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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Epigenetic Regulation01:46

Epigenetic Regulation

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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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相关实验视频

Updated: Jan 13, 2026

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
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多种模式的bHLH-PASDNA结合控制了特异性,并导致肥胖.

David C Bersten1,2, Daniel P McDougal1,3, Adrienne E Sullivan1,4,5

  • 1The Department of Molecular and Biomedical Science, School of Biological Sciences, The University of Adelaide, Adelaide, SA 5001, Australia.

Nucleic acids research
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概括

基本螺旋-循环-螺旋 Per-Arnt-Sim (PAS) 转录因子 (TF) 家族

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Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物化学 生物化学

背景情况:

  • 基本螺旋环螺旋Per-Arnt-Sim (bHLH-PAS) 转录因子 (TF) 家族是生理和环境反应的关键调节者.
  • 对于bHLH-PAS TFs的DNA结合和目标基因调节的特异性机制尚未完全理解.

研究的目的:

  • 系统地分析bHLH-PAS TF家族关键成员的DNA结合层次和特异性机制.
  • 阐明DNA形状和侧面序列在TF结合特异性中的作用.
  • 研究SIM1 PAS循环/DNA相互作用在TF活动和疾病中的功能意义.

主要方法:

  • 对原型bHLH-PAS TFs (ARNT,ARNT2,HIF1α,HIF2α,AhR,NPAS4,SIM1) 的相关DNA结合层次的系统分析.
  • 研究DNA结合足迹和序列偏好.
  • 对TF-DNA相互作用的分析,包括DNA形状和侧面序列贡献.
  • 在SIM1.R171H敲进小鼠模型中的功能研究.

主要成果:

  • 确定了bHLH-PAS TFs的大型DNA结合足迹 (12-15 bp).
  • 揭示了侧翼编码的DNA结合特异性,通过N端相互作用区分SIM1和HIFTF结合.
  • 证明了DNA形状,核心和侧翼TF结合之间的关系,使动机序列灵活性.
  • 发现了新的SIM1 PAS-loop/DNA相互作用与AT丰富的序列,对DNA结合和转录活性至关重要.
  • 在小鼠模型中确定了与SIM1功能相关的超性肥胖的单一原因.

结论:

  • bHLH-PAS TF结合特异性是通过涉及核心动机,侧面序列和DNA形状的多式机制实现的.
  • SIM1 PAS-循环/DNA相互作用对TF功能至关重要,并与过性肥胖症有关.
  • 这项研究解决了bHLH-PAS TF DNA结合特异性的关键方面及其对恒温和疾病的影响.