Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.3K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.3K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.3K
2.3K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

5.3K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
5.3K
Homologous Recombination02:31

Homologous Recombination

62.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.6K
Homologous Recombination02:31

Homologous Recombination

6.2K
6.2K
The DNA Replication Fork01:02

The DNA Replication Fork

40.5K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

PARP1 trapping activates ATM-mediated NF-κB signaling independent of replication in response to TOP1 blockade.

Nucleic acids research·2026
Same author

Condensin I but not Condensin II is crucial for mitotic chromosome mechanics.

Nature communications·2026
Same author

DNA secondary structures in BCL2 and MYC elicit activation-induced cytidine deaminase binding and activity.

Nucleic acids research·2026
Same author

<i>HDAC5</i>-encoded Microprotein NISM Mediates Nucleolar Formation and Ribosomal RNA Synthesis.

bioRxiv : the preprint server for biology·2026
Same author

circPCMTD1: a protein-coding circular RNA that regulates DNA damage response in BCR/ABL1-positive leukemias.

Blood·2026
Same author

SMC5/6-mediated plasmid silencing is directed by SIMC1-SLF2 and antagonized by the SV40 large T antigen.

eLife·2025
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
查看所有相关文章

相关实验视频

Updated: Jan 17, 2026

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.6K

SMC5/SMC6复合体对于解决R循环诱导的转录复制冲突至关重要.

Tong Wu1, Youhang Li1,2,3, Yuqin Zhao1

  • 1Department of Molecular and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037,United States.

Nucleic acids research
|January 14, 2026
PubMed
概括
此摘要是机器生成的。

SMC5/6复合物解决了威胁基因组稳定的转录复制冲突 (TRCs). 这一发现揭示了治疗缺乏毒素 (SETX) 的瘤的新途径.

更多相关视频

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K
Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
08:24

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

1.7K

相关实验视频

Last Updated: Jan 17, 2026

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.6K
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K
Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
08:24

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Published on: July 27, 2021

1.7K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 基因组学就是基因组学.

背景情况:

  • R环对细胞功能至关重要,但可以通过引起转录复制冲突 (TRC) 来破坏基因组的稳定.
  • 参素 (SETX) 是一种RNA/DNA螺旋酶,在复制过程中分解R循环.

研究的目的:

  • 调查SMC5/6复合体在解决TRC和保持基因组稳定中的作用.
  • 确定SMC5/6在TRC分辨率中运行的分子机制.

主要方法:

  • 研究了SMC5/6和SETX之间的合成致命相互作用.
  • 利用基于细胞的测试来追踪蛋白质复合物的招募到TRCs.
  • 分析了TRC解决途径的功能后果.

主要成果:

  • 在缺乏毒素的细胞中,SMC5/6复合体被招募到TRC中,感知DNA超级卷.
  • SMC5/6促进了BLM/TOP3A/RMI1/RMI2 (BTRR) 综合体的招聘,以解决TRCs.
  • SMC5/6-BTRR轴与FANCM和FANCD2一起,减轻了TRC诱导的基因组不稳定性.

结论:

  • SMC5/6复合体在感知和解决TRC方面发挥着至关重要的作用.
  • 一个新的SMC5/6-BTRR-FANCM-FANCD2通路被定义用于缓解基因组不稳定性.
  • 针对这一轴为缺乏SETX的瘤提供治疗潜力.