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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Complementary DNA

Complementary DNA

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Genomic DNA in Eukaryotes

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.

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相关实验视频

Updated: Jan 18, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

潘塞翁-DNA:多功能编码解码系统,集成适应性NGS预处理算法,用于DNA数据存储.

Adriano Galindo Leal¹, Thiago Yuji Aoyagi¹, André Guilherme Costa-Martins¹

¹Artificial Intelligence and Analytics Department, Institute for Technological Research, São Paulo, 05508-901, SP, Brazil.

Computational and structural biotechnology journal

|January 16, 2026

概括

此摘要是机器生成的。

Pantheon-DNA是DNA数据存储的新管道,可以高效地处理大型数据集. 它实现了高数据可检索性,并使用新的方法来防止DNA序列编码和检索过程中的错误.

关键词:

DNA数据存储 DNA数据存储编码方案编码方案序列数据预处理序列数据预处理软件工程软件工程软件工程

更多相关视频

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

相关实验视频

Last Updated: Jan 18, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

科学领域:

生物技术是生物技术.
生物信息学是一种生物信息学.
数据存储数据存储数据存储

背景情况:

DNA数据存储提供了高密度,但面临着可扩展性和错误挑战.
重复的DNA序列可能会导致分子级别的错误,并使数据处理复杂化.
从DNA中有效管理和检索大型数据集对于实际应用至关重要.

研究的目的:

介绍Pantheon-DNA,一个端到端的处理管道用于DNA数据存储.
解决DNA数据存储系统的可扩展性和效率挑战.
提高基于DNA的数据存储中的数据可检索性和可靠性.

主要方法:

开发了一个数据排列方案和随机化程序,以防止有问题的DNA序列模式.
实现了块数据架构,以增强并行处理和检索.
利用编码数据结构的先前知识来简化预处理和减少计算复杂性.

主要成果:

在低错误率 (LER) 和高错误率 (HER) 条件下,在10倍覆盖率下实现≥99.996%的数据可检索性.
在合成和测序实验中,成功编码和解码了1.59 MB的数据,其中包含多个文件.
通过预处理管道演示了减少计算复杂性和简化集群程序.

结论:

潘提昂-DNA有效地解决了DNA数据存储的可扩展性和效率.
提出的方法提高了通过实验结果验证的数据可靠性和可检索性.
未来的工作重点是改进错误纠正,特别是用于indel恢复,并优化预处理.