Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.3K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.3K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.3K
2.3K
The DNA Replication Fork01:02

The DNA Replication Fork

40.5K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.5K
The DNA Replication Fork01:02

The DNA Replication Fork

18.2K
18.2K
Replication in Eukaryotes01:29

Replication in Eukaryotes

17.1K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
17.1K
Replication in Eukaryotes02:31

Replication in Eukaryotes

203.5K
Overview
203.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The evolving landscape of enzymatic technologies for precision synthesis of antibody-drug conjugates.

Antibody therapeutics·2026
Same author

Inhibition of ADSS2-mediated de novo AMP biosynthesis re-sensitizes acute myeloid leukemia to BH3 mimetics.

Nature cancer·2026
Same author

Human RIG-I Antiviral Deficiency Caused by a Dominant-Negative Variant Locked in a Signaling-Inactive State.

medRxiv : the preprint server for health sciences·2026
Same author

Synthesis of Bispecific Conjugates by ADP-Ribosyl Cyclases.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Structural determinants of non-covalent PPARγ inverse agonism and their therapeutic implications.

Nature communications·2025
Same author

A versatile platform for chemical engineering of exosomes empowered by ADP-ribosyl cyclases.

Nature communications·2025
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jan 18, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K

PAR驱动的冷凝保持了停滞的复制叉的稳定性.

Lei Zhang, Zeyu Zhang, Timothy R O'Leary

    bioRxiv : the preprint server for biology
    |January 16, 2026
    PubMed
    概括
    此摘要是机器生成的。

    当蛋白质酶被抑制时,聚ADP-ribose (PAR) 形成核凝结物,与全域链和蛋白质酶一起凝结. 这些结构通过稳定停滞的复制分叉来保护基因组完整性.

    更多相关视频

    Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
    05:22

    Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

    Published on: September 13, 2024

    1.2K
    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
    07:18

    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

    Published on: October 27, 2011

    40.6K

    相关实验视频

    Last Updated: Jan 18, 2026

    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
    07:27

    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

    Published on: April 29, 2010

    14.0K
    Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
    05:22

    Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

    Published on: September 13, 2024

    1.2K
    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
    07:18

    Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

    Published on: October 27, 2011

    40.6K

    科学领域:

    • 细胞生物学 细胞生物学
    • 生物化学 生物化学
    • 基因组学就是基因组学.

    背景情况:

    • 聚ADP-ribose (PAR) 是一种类似于核酸的聚合物,参与细胞事件.
    • PAR聚合酶 (PARP) 催化 PAR 形成作为一个翻译后的修饰.
    • 液-液相分离 (LLPS) 生成功能性亚细胞凝结物.

    研究的目的:

    • 研究PAR在生物分子凝聚中的作用.
    • 为了确定细胞条件和影响PAR凝结的因素.
    • 了解PAR凝结在应激细胞中的功能影响.

    主要方法:

    • 基于小分子的成像查.
    • 在各种细胞类型中抑制蛋白酶体.
    • 对 PAR,蛋白质酶和泛素链的同局部化研究.
    • 对DNA复制叉稳定性的评估.

    主要成果:

    • 在蛋白质酶抑制后,PAR经历LLPS,形成核凝结物.
    • 这些凝结物涉及PAR,蛋白酶和泛素链,取决于PARP2和K6相关的泛素化.
    • PAR首次直接与乌比奎丁链相互作用.
    • 停滞的DNA复制分叉与这些凝聚物共同定位.

    结论:

    • PAR凝结是细胞在蛋白质体应激下的一种新型自我保护机制.
    • PAR-proteasome-ubiquitin链凝聚剂稳定了停滞不前的复制分叉,保持了基因组的完整性.
    • 这项研究提供了对PAR凝结及其细胞功能的基本见解.