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相关概念视频

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

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The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
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Genomic Imprinting and Inheritance02:30

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Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
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Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

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While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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Tonicity in Animals00:59

Tonicity in Animals

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The tonicity of a solution determines if a cell gains or loses water in that solution. The tonicity depends on the permeability of the cell membrane for different solutes and the concentration of nonpenetrating solutes in the solution within and outside of the cell. If a semipermeable membrane hinders the passage of some solutes but allows water to follow its concentration gradient, water moves from the side with low osmolarity (i.e., less solute) to the side with higher osmolarity (i.e.,...
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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
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教程:动物基因组的注释

Zoe A Clarke1,2, Dustin J Sokolowski1,3, Ciaran K Byles-Ho4

  • 1Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.

Nature protocols
|January 28, 2026
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概括

本教程介绍了一个精简的基因组注释管道,用于创建高质量的动物基因组注释. 它整合了基因预测,功能证据和重复区域注释的先进工具,提高了基因组研究的可访问性.

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科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 在DNA测序方面的进步有助于从非模型生物中组装新的基因组.
  • 基因组注释,即识别基因和其他特征的过程,对于利用新组装的基因组序列作为参考来说至关重要.
  • 现有的注释管道在准确性,资源需求和可用性方面各不相同.

研究的目的:

  • 描述一条精简的基因组注释管道,用于在实验室环境中制作高质量的动物基因组注释.
  • 整合最先进的工具来注释蛋白质编码和非编码RNA基因.
  • 为了指导用户分配基因符号,注释重复区域,并评估注释质量.

主要方法:

  • 整合现有的,最先进的基因组注释工具.
  • 包括从组装的DNA序列中基因预测.
  • 纳入基因同质信息和功能证据 (蛋白质序列,RNA测序数据).

主要成果:

  • 一个精简的工作流程,用于高质量的动物基因组的基因组注释.
  • 关于基因符号分配和重复区域注释的指导.
  • 评估和格式化注释结果的工具描述.

结论:

  • 描述的管道为实验室提供了一种实用的方法,可以生成可靠的基因组注释.
  • 这种工作流程增强了新测序的动物基因组在研究中的实用性.
  • 该教程旨在提高基因组注释过程的可访问性和质量.