Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Editing02:23

RNA Editing

9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
Optimal Foraging00:48

Optimal Foraging

13.9K
How animals obtain and eat their food is called foraging behavior. Foraging can include searching for plants and hunting for prey and depends on the species and environment.
13.9K
Overview of Advanced Functional Groups02:22

Overview of Advanced Functional Groups

30.1K

Functional groups are groups of atoms with specific chemical properties that occur within organic molecules and are sometimes denoted as “R”. Functional groups can “functionalize” a compound by enabling it to adopt different physical and chemical properties.
Types of Advanced Functional Groups
The table below summarizes some of the major functional groups in organic chemistry.
30.1K
Optimization Problems01:26

Optimization Problems

75
Optimization problems often involve identifying maximum or minimum values under specific constraints. A well-known example is determining the longest horizontal pipe that can be moved around a right-angled corner, where a 3-meter-wide hallway meets a 2-meter-wide hallway. This scenario, common in architectural design and industrial transport, can be understood conceptually through geometric and trigonometric reasoning.To visualize the problem, consider the pipe as a straight line that touches...
75
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

1.2K
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
1.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Massive pulmonary embolism after cesarean section in a pregnant with occult pneumonia after COVID-19 infection: A case report.

Medicine·2025
Same author

Nationwide analysis of routine clinical practices in the management of acute ischemic stroke patients in China.

International journal of stroke : official journal of the International Stroke Society·2025
Same author

The Radiosensitizing Effect of Tumor-Derived Microparticles Co-Loaded with Sorafenib and Gold Nanoparticles on Hepatocellular Carcinoma.

International journal of nanomedicine·2025
Same author

Behavior recognition technology based on deep learning used in pediatric behavioral audiometry.

Scientific reports·2025
Same author

LianChuang ZhiXue Liquid Enema Modulates the Macrophage Polarization of Ulcerative Colitis via Inhibiting the Jagged-1/Notch1 Signaling Pathway.

Drug design, development and therapy·2025
Same author

Regulatory T cell depletion promotes myeloid cell activation and glioblastoma response to anti-PD1 and tumor-targeting antibodies.

Immunity·2025

相关实验视频

Updated: Feb 6, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.8K

优化主要编辑:提高效率提升方面的进步

Ying Qu1, Yingying Li2, Tong Shao2

  • 1College of Science, National University of Defense Technology, Changsha 410073, China.

Biotechnology advances
|February 4, 2026
PubMed
概括
此摘要是机器生成的。

主编辑 (PE) 提供了精确的基因组工程,没有双链断裂. 蛋白质工程,pegRNA设计和准策略的最新进展显著提高了PE的效率并扩大了其应用.

关键词:
编辑效率提高了编辑效率.板平衡平衡的方法不匹配修复路径的修复路径PAM兼容性兼容性的问题主要编辑主要编辑蛋白质工程是一种蛋白质工程.pegRNA工程 pegRNA工程是什么

更多相关视频

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.1K
Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

7.0K

相关实验视频

Last Updated: Feb 6, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.8K
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.1K
Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

7.0K

科学领域:

  • 分子生物学分子生物学
  • 基因组工程是基因组工程.
  • 生物技术是生物技术.

背景情况:

  • 主编辑 (PE) 是一种强大的基因组编辑技术.
  • 它允许精确的插入,删除和基础转换.
  • 目前的PE效率是可变的,并且取决于环境.

研究的目的:

  • 审查和巩固近期在原始编辑技术的进步.
  • 为了说明综合增强如何克服效率瓶.
  • 突出优化PE在各种生物应用中的潜力.

主要方法:

  • 为了提高活动和稳定性,主要编辑器变体的蛋白质工程.
  • 在pegRNA设计中进行结构改进,以提高完整性和抗性.
  • 规范主要编辑不匹配修复 (MMR) 过程的策略.
  • 为了扩展准,开发了原体空间体相邻动机 (PAM) 放松的Cas变体.

主要成果:

  • 优化的主要编辑器变体显示了增强的逆转录酶活性.
  • 改进的pegRNA设计增加了功能完整性并减少了降解.
  • MMR路径调节促进了所需的编辑保留.
  • PAM放松的Cas变体扩大了可向基因组位点的范围.

结论:

  • 整合结构性,机械性和定位性增强可以克服主要编辑瓶.
  • 优化原始编辑是一种用于精密基因组工程的多功能和高效系统.
  • 这些发展促进了主要编辑在各种生物环境中的可靠应用.