工程化益生菌可以在小鼠的胃肠道系统中隔离化物
bioRxiv : the preprint server for biology
|February 6, 2026
概括
工程化益生菌可以在肠道中拦截有毒的酸盐,防止其吸收到血液中. 这种合成生物学方法提供了一种新的策略,以减轻受污染的食物和水对健康的风险.
科学领域:
- 合成生物学 合成生物学
- 微生物治疗药物 微生物治疗药物
- 环境毒理学环境毒理学
背景情况:
- 在食物和水中长期暴露于无机 () 是一个全球性的健康问题.
- 酸盐很容易被吸收,并在组织中积累,导致癌症,器官功能障碍和神经系统疾病.
- 目前的策略缺乏切实可行的方法来防止饮食中矿在胃肠道 (GI) 吸收.
研究的目的:
- 开发一种基于合成生物学的方法,使用工程益生菌来隔离肠道内的化物.
- 创建一个活的化物拦截系统,以防止饮食中毒物的系统吸收.
- 建立一个可编程的益生菌平台,以减轻化物暴露.
主要方法:
- 工程化Escherichia coli Nissle 1917 (EcN) 具有对素敏感的遗传切换开关和用于隔离的修饰素结合蛋白 (ArsR).
- 开发了一种质量转移模型来预测体内应用的最佳细菌剂量.
- 在小鼠胃肠道模型中测试了工程EcN菌株,以评估酸盐吸收减少.
主要成果:
- 工程 EcN 在实验室中有效地去除了化物,同时保持了细胞活力和生长.
- 质量转移模型指导了选择一种细菌剂量的指导,该剂量显著降低了光质酸盐.
- 与对照组相比,在小鼠中口服工程EcN显著减少了矿进入血液的数量.
结论:
- 工程化益生菌可以有效地在消化道内拦截饮食中含有的化物.
- 这种可编程益生菌平台展示了防止环境毒素吸收的潜在策略.
- 活微生物疗法提供了一种新的方法来打击与污染相关的健康风险.
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