相关实验视频

Updated: Feb 7, 2026

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

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在IAL-PiD2昆虫细胞中通过反应剂传递优化和细胞同步增强CRISPR同质定向修复

Bryce D Shirk1, Cecilia Z Rodriguez2, Marisa O Pacheco2

  • 1J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, 32611.

Biochemical engineering journal
|February 6, 2026
PubMed
概括

这项研究在昆虫细胞中建立了CRISPR/Cas9同质导向修复 (HDR),用于精确的基因组编辑. 优化条件和细胞周期同步显著提高了非病毒基因传递的HDR效率.

关键词:
这就是CRISPR/Cas9的作用.同质导向维修 (HDR) 是指同质导向维修.昆虫的细胞是昆虫的细胞.细胞同步的同步.基因组编辑 基因组编辑基尿素的含量是多少

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