Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Other Unique Bacteria01:18

Other Unique Bacteria

449
Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic...
449
Second Uniqueness Theorem01:16

Second Uniqueness Theorem

2.7K
Consider a region consisting of several individual conductors with a definite charge density in the region between these conductors. The second uniqueness theorem states that if the total charge on each conductor and the charge density in the in-between region are known, then the electric field can be uniquely determined.
In contrast, consider that the electric field is non-unique and apply Gauss's law in divergence form in the region between the conductors and the integral form to the surface...
2.7K
Improving Translational Accuracy02:07

Improving Translational Accuracy

15.0K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
15.0K
Improving Translational Accuracy02:07

Improving Translational Accuracy

3.7K
3.7K
Uncertainty in Measurement: Accuracy and Precision03:37

Uncertainty in Measurement: Accuracy and Precision

102.7K
Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value. 
102.7K
Accuracy and Precision01:52

Accuracy and Precision

15.5K
Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value.  Highly accurate...
15.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Systematic decoding the functional role of human endogenous retrovirus-derived RNAs in medulloblastoma.

Neuro-oncology advances·2026
Same author

TEITbase: a database for transposable element (TE)-initiated transcripts in human cancers.

Database : the journal of biological databases and curation·2026
Same author

Locus-Specific Human Endogenous Retrovirus ERVK18 Expression Indicates an Inflamed Microenvironment and Favorable Immunotherapy Outcome in Small Cell Lung Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Molecular landscape, subtypes, and therapeutic vulnerabilities of central nervous system solitary fibrous tumors.

Nature communications·2025
Same author

Common signatures of neutrophils in diverse disease conditions.

Cell discovery·2025
Same author

Functional diversification of the MADS-box gene family in fine-tuning the dimorphic transition of <i>Talaromyces marneffei</i>.

mSystems·2025

相关实验视频

Updated: Feb 8, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

2.0K

放大优化和独特的分子标识器引导的高精度全长圆形RNA测序.

Yueqi Jin1, Xueyan Hu1,2, Yun Zhang1

  • 1Department of Medical Bioinformatics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.

Genomics, proteomics & bioinformatics
|February 6, 2026
PubMed
概括

这项研究介绍了ucircFL-seq,这是一种新的测序方法,可以提高循环RNA (circRNA) 识别和量化的准确性. 这一进步有助于解决测序平台之间的不一致性,以便更好地进行circRNA研究.

关键词:
一个全长的圆形RNA.长读序列的测序方式独特的分子标识符.环RNA 环RNA 是一个环RNA.在ucircFL-seqqq中使用.

更多相关视频

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

12.8K
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
10:18

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

12.7K

相关实验视频

Last Updated: Feb 8, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

2.0K
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

12.8K
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
10:18

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

12.7K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 在RNA生物学,RNA生物学.

背景情况:

  • 循环RNA (circRNAs) 是调控性非编码RNA,在生理过程和疾病中起着关键作用.
  • 准确识别和定量circRNAs对于理解它们的功能和临床相关性至关重要.
  • 现有的高通量测序工作流显示出不一致性,部分原因是图书馆准备错误.

研究的目的:

  • 调查circRNA识别工作流程中不一致的原因.
  • 为准确的全长circRNA测序和定量开发一种改进的方法.
  • 在circRNA检测中增强跨平台一致性.

主要方法:

  • 建立了一个基于UMI的全长circRNA测序方法 (ucircFL-seq).
  • 在测序工作流程中优化信号放大程序.
  • 使用独特的分子标识符 (UMI) 来提高准确性.

主要成果:

  • 在图书馆准备过程中确认的序列错误有助于工作流不一致.
  • ucircFL-seq显著提高了circRNA检测和量化的准确性.
  • 实现了更强大的跨平台对应性,用于circRNA识别.
  • 在不同长度和结构的平台之间确定了不同的circRNA池.

结论:

  • ucircFL-seq提供了一种以UMI为指导的方法,以提高全长circRNA识别和量化准确度.
  • 这些发现表明,不同测序平台在circRNA发现中具有互补作用.
  • 这种方法有助于进一步探索circRNAs的功能.