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相关概念视频

Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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Histone Modification02:32

Histone Modification

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Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

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The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
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Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
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Post-translational Translocation of Proteins to the RER01:27

Post-translational Translocation of Proteins to the RER

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A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
7.8K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

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Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
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通过光谱流细胞测量,在整个细胞中对核素3的翻译后修饰进行分析.

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    此摘要是机器生成的。

    通过使用光谱流细胞计,EpiFlow可以在整个细胞中对基因组翻译后修饰 (PTM) 进行多参数分析. 这种方法提高了吞吐量,并保持了样本的完整性,以便全面了解染色体动态.

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    Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
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    科学领域:

    • 分子生物学分子生物学
    • 细胞生物学 细胞生物学
    • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

    背景情况:

    • 基因组基因修饰调节基因表达和细胞身份.
    • 单独研究各种基因组翻译后修饰 (PTMs) 限制了对它们协调作用的理解.
    • 传统的子PTM流细胞计具有较低的多重复合能力,需要核隔离,导致样本损失.

    研究的目的:

    • 开发一种新的光谱流细胞计协议,EpiFlow,用于在整个细胞中对基因组PTM进行多参数分析.
    • 与传统方法相比,提高吞吐量,效率和样本完整性.
    • 为研究色素动态提供一种可访问和可重复的方法.

    主要方法:

    • 开发了EpiFlow,这是一种用于全细胞组质子PTM分析的光谱流细胞计协议.
    • 使用96井板格式来提高吞吐量.
    • 在整个分析过程中保持细胞完整性.

    主要成果:

    • 在神经前细胞内,EpiFlow成功地解决了组织素PTM的微妙变异.
    • 该方法在整个细胞周期中捕获了不同的染色质状态.
    • 结果与几个细胞标记物相关联,证明了全面的细胞状态分析.

    结论:

    • EpiFlow提供了一个强大的框架,用于探索染色质动态和基因素PTMs.
    • 该协议通过多参数分析增强了对细胞身份和功能的理解.
    • 开放获取资源确保在基础和治疗研究中的可重复性和广泛适用性.