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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

19.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.2K
Genetic Screens02:46

Genetic Screens

5.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.8K
Homologous Recombination02:31

Homologous Recombination

63.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
63.7K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.0K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.0K
CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

12.0K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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相关实验视频

Updated: Feb 17, 2026

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

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为更好的预测提供更好的数据:数据策划改善了对sgRNA/Cas9预测的深度学习.

Tyler S Browne1, David R Edgell1, Gregory B Gloor1

  • 1Department of Biochemistry, Schulich School of Medicine & Dentistry, University of Western Ontario, London, Ontario, Canada.

PeerJ
|February 16, 2026
PubMed
概括

优化CRISPR-Cas9 (CRISPR相关蛋白9) 模型的数据输入可以提高抗菌应用的预测准确性. 这种以数据为中心的方法提高了跨细菌物种的Cas9预测模型的实用性.

关键词:
细菌CRISPR预测的方法这是一个CRISPR模型.在CRISPR的预测过程中.数据的清理数据的清理.高吞吐量测序的测序方法机器学习 机器学习模型建筑模型建筑SgRNA活动预测预测.

更多相关视频

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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相关实验视频

Last Updated: Feb 17, 2026

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因工程是一种基因工程.

背景情况:

  • Cas9酶和单导向RNA (sgRNA) 是基因工程中的关键工具,显示为特定物种的抗微生物药物的潜力.
  • 准确预测目标Cas9裂变对于其抗菌疗效至关重要,因为活动在目标之间有很大差异.
  • 以前的研究集中在新的深度学习架构上,以提高预测性能.

研究的目的:

  • 研究以数据为中心的方法对改善Cas9预测模型的影响.
  • 通过调整目标站点相邻的核酸序列长度和过控制读数来优化输入数据的实用性.
  • 使用 crisprHAL 架构开发一个通用的细菌 SpCas9 预测模型.

主要方法:

  • 探索了以数据为中心的策略,包括优化输入序列长度和对读数进行数据过.
  • 利用现有的crisprHAL深度学习架构开发了一个新的细菌SpCas9预测模型 (crisprHAL Tev).
  • 重建了之前的两个大肠杆菌Cas9数据集,以评估数据质量的影响.

主要成果:

  • 以数据为中心的方法,特别是输入优化和过,显著改善了Cas9预测的输入数据实用性.
  • 开发的 crisprHAL Tev 模型在相关的细菌物种和不同数据类型中展示了通用的性能.
  • 重建E.E.的重建工作 大肠杆菌数据集强调了数据质量的关键作用,从而改善了细菌eSpCas9预测模型.

结论:

  • 以数据为中心的方法对于提高Cas9预测模型的性能和通用性至关重要.
  • crisprHAL Tev模型为细菌SpCas9活动提供了更好的预测准确性,适用于各种物种.
  • 数据质量至关重要;改进的数据集有助于对基于CRISPR的抗微生物药物的更强大,更可靠的预测模型.