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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Feb 19, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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FragmentFinder-一个用户友好的,基于Windows的工具,用于识别和表征从任何非编码RNA中切除的短RNA.

Jeffrey D DeMeis1, Courtlyn M Breaux2, Allison H Powell2

  • 1Department of Pharmacology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL 36688, United States.

NAR genomics and bioinformatics
|February 18, 2026
PubMed
概括

研究人员开发了FragmentFinder,这是一个用户友好的工具,用于识别和表征短非编码RNA (sncRNA) 片段. 这个平台从测序数据中分析所有sncRNA衍生RNA,包括新片段,而不需要计算专业知识.

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相关实验视频

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科学领域:

  • * 分子生物学 * 分子生物学
  • * 基因组学 是一个学科.
  • * 生物信息学是一门学科.

背景情况:

  • * 短非编码RNAs (sncRNAs) <200核酸调节细胞活动.
  • * 微RNA (miRNA) 是参与基因调节的关键类型的sncRNA.
  • *新型调节性sncRNA片段在分析中经常被忽视.

研究的目的:

  • * 开发一个用户友好的平台,用于识别和表征sncRNA片段.
  • * 为了分析注释和未知的sncRNA衍生RNAs.
  • * 克服sncRNA片段分析中的计算障碍.

主要方法:

  • * FragmentFinder的开发,这是一个Windows可执行的软件.
  • *从原始小RNA测序数据中实时表征sncRNA衍生的RNA.
  • * 专为具有最低编程经验的用户而设计.

主要成果:

  • * FragmentFinder准确地描述了所有sncRNA衍生RNA,包括新型碎片.
  • * 该平台不需要计算背景才能运行.
  • * 能够对sncRNA表达特征进行全面分析.

结论:

  • * FragmentFinder为sncRNA片段分析提供了一个可访问的解决方案.
  • * 有助于研究以前未经表征的sncRNA片段.
  • *促进了对调节性RNA生物学的理解.