Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Distinct energetic blueprints diversify function of conserved protein folds.

Nature chemistry·2026

Same author

Human RIG-I Antiviral Deficiency Caused by a Dominant-Negative Variant Locked in a Signaling-Inactive State.

medRxiv : the preprint server for health sciences·2026

Same author

A hotspot phosphorylation site on SHP2 drives oncoprotein activation and drug resistance.

Nature communications·2026

Same author

Predictive value of the combined application of oxidative stress indicators and carcinoembryonic antigen for patients with early lung adenocarcinoma.

American journal of translational research·2026

Same author

Bidirectional allosteric ligand regulation in a central glycolytic enzyme.

bioRxiv : the preprint server for biology·2026

Same author

HXMS: a standardized file format for HX/MS data.

bioRxiv : the preprint server for biology·2025

Search research articles

相关实验视频

Updated: Mar 11, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

Published on: May 4, 2020

HXMS:用于HX-MS数据的标准化文件格式.

Kyle C Weber¹, Chenlin Lu¹, Roberto Vera Alvarez²

¹Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.

Bioinformatics (Oxford, England)

|March 10, 2026

概括

此摘要是机器生成的。

一个新的文件格式,HXMS和一个Python包,PFLink,标准化/交换质谱 (HX-MS) 数据. 这使得更多的定量分析和数据共享可用于蛋白质构成研究.

更多相关视频

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

Published on: June 23, 2022

Quantifying X-Ray Fluorescence Data Using MAPS

Quantifying X-Ray Fluorescence Data Using MAPS

Published on: February 17, 2018

相关实验视频

Last Updated: Mar 11, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

Published on: May 4, 2020

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

Published on: June 23, 2022

Quantifying X-Ray Fluorescence Data Using MAPS

Quantifying X-Ray Fluorescence Data Using MAPS

Published on: February 17, 2018

科学领域:

生物化学生物化学
结构生物学结构生物学
计算生物学计算生物学

背景情况:

/交换质谱 (HX-MS) 对于研究蛋白质构成组件至关重要.
目前的HX-MS数据分析是不一致的,因为不同的软件和非标准化的格式.
现有的格式经常使用平均化,失去有价值的同位素信息.

研究的目的:

为HX-MS数据引入一个统一,可扩展和人类可读的文件格式 (HXMS).
开发一个Python包 (PFLink) 用于将现有的HX-MS数据转换为新格式.
增强定量分析,数据共享和未来的应用,如HX-MS中的机器学习.

主要方法:

开发了HXMS文件格式,灵感来自蛋白质结构和基因组学标准.
创建了PFLink,这是一个Python包,用于将常见的HX-MS软件中的数据转换为HXMS.
确保HXMS保存同位素质量包裹,时间流程数据和实验细节.

主要成果:

该HXMS格式支持完整的同位素包裹,时间过程数据,PTM和复制品.
PFLink 便于无转换来自各种 HX-MS 软件的数据.
这些组合的工具可以实现更高分辨率的定量分析和改进的数据管理.

结论:

该HXMS格式和PFLink包解决了HX-MS中关键的标准化需求.
这些工具将在现场推进定量分析,数据共享和计算方法.
促进HX-MS技术的更广泛采用和进一步发展.