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相关概念视频

RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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RNA Interference01:23

RNA Interference

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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

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The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
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相关实验视频

Updated: Mar 15, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

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可编程RNA N6,2'-O-Dimethyladenosine编辑 编辑

Yang Li1, Xiangmin Tan2, Yaran Liu3

  • 1Genetic Diseases Key Laboratory of Sichuan Province, Department of Medical Genetics, Department of Laboratory Medicine, School of Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|March 13, 2026
PubMed
概括

研究人员开发了针对性m6Am甲基化 (TAmM),一种新的RNA编辑工具. 这个平台精确地将N6,2′-O-dimethyladenosine (m6Am) 修改添加到特定的RNA转录中,使得对基因调节和翻译的新见解成为可能.

关键词:
在CRISPR-Cas13中使用.N6,2 ́-O-dimethyladenosine是指一种甲基氨酸的类型.编辑RNA的RNA编辑基因组RNA的修饰是RNA的修饰.

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

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相关实验视频

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
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科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • N6,2′-O-dimethyladenosine (m6Am) 是一种关键的RNA修饰,影响基因调节.
  • 现有的方法缺乏准确性来研究m6Am的特定功能.

研究的目的:

  • 开发一个可编程的平台,用于特定站点的m6Am安装在细胞转录上.
  • 研究m6Am在基因表达和转换中的功能性作用.

主要方法:

  • 设计了一种融合蛋白,结合了催化不活的RfxCas13d (dCasRx) 和m6Am甲基转移酶PCIF1.1.
  • 利用TAmM平台以单核酸分辨率进行向的m6Am沉积.
  • 使用LC-MS/MS验证的m6Am安装,以及对mRNA,蛋白质水平和多元体关联的评估影响.

主要成果:

  • 实现高保真性高效和特定的m6Am安装.
  • 证明有针对性的m6Am编辑调节蛋白质输出而不会改变mRNA丰度.
  • 显示的m6Am安装增强了多体关联,影响了翻译调节.
  • 证实m6Am沉积在CTNNB1上有助于癌细胞的增殖和迁移.

结论:

  • TAmM是一个精确和多功能平台,用于可编程的m6Am操纵.
  • 揭示了m6Am在基因调节和癌症进展中的转录特异性作用.
  • 建立了一个新的工具来剖析RNA修饰的功能影响.