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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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挖掘多能性:纳米注射到人类iPSC中的方法

Jann Harberts1,2, Vuong Thi Thanh Xuan Ho1,2, Yuqi Yang2,3

  • 1Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.

Advanced materials (Deerfield Beach, Fla.)
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概括

纳米管纳米注射提供了一种安全,高效的非病毒方法,用于将信使RNA (mRNA) 输送到人类诱导多能干细胞 (hiPSC) 中. 这种技术保持了hiPSC多能性和差异化能力,推进了再生医学和疾病建模应用.

关键词:
人类诱导的多能干细胞干细胞.它们是mRNARNA.纳米注射纳米注射的方法尼纳尼德尔 ( nanoneedles) 是一种纳米管是一种纳米管转化过程中的转化.

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科学领域:

  • 生物技术和再生医学 生物技术和再生医学
  • 细胞工程 细胞工程
  • 纳米技术纳米技术

背景情况:

  • 人类诱导的多能干细胞 (hiPSCs) 对再生医学和疾病建模至关重要.
  • 目前的遗传物质传递方法 (病毒,电穿孔) 有安全性,精度和细胞毒性限制.
  • 将外源遗传物质有效和安全地输送到hiPSC中,对于它们的应用至关重要.

研究的目的:

  • 引入纳米管 (NT) 介导的纳米注射作为一种新的非病毒战略,用于将功能信使RNA (mRNA) 输送到hiPSC中.
  • 为了证明NT介导的纳米注射在hiPSCs中的有效性和安全性,保持它们的多能状态.

主要方法:

  • 开发了经过重新设计的NT,具有增强的载荷能力和细胞接口几何.
  • 实施延迟细胞外矩阵 (ECM) 应用和先进的表面工程,用于hiPSC纳米注射.
  • 通过纳米注射将各种mRNA有效载荷 (mCherry,GFP,YPet) 输送到hiPSC中.

主要成果:

  • 在hiPSC中实现了有效的纳米注射,单个mRNA的平均转染产量为~55%~64%.
  • 证明了mCherry和GFPmRNA的成功共传染,产量为61%左右.
  • 在纳米注射后通过标记表达 (NANOG,OCT4,SOX2) 和神经元在几个通道上的分化能力确认了hiPSC完整性和多能性.

结论:

  • 通过NT介导的纳米注射是一种强大而有效的非病毒工具,用于将mRNA输送到hiPSC中.
  • 这种方法保持了hiPSC多能性和功能差异化能力.
  • 纳米注射作为一个启用平台,用于精确的细胞工程和先进的干细胞应用.