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Updated: Mar 16, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
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Published on: May 5, 2018

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从小型活检和细胞样本进行RNA测序的分析前最佳实践.

Gloria Hopkins Sura1, Kelly B Engel2, Sarah R Greytak3

  • 1Division of Pathology and Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. ghsura@mdanderson.org.

Molecular diagnosis & therapy
|March 14, 2026
PubMed
概括

来自微侵袭性癌症样本的RNA测序对于精确瘤学至关重要. 本综述概述了样本处理的最佳实践,以确保可靠的RNA测序结果,改善患者护理和研究.

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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