Related Experiment Videos
[Experimental pulmonary emphysema in rats. Inflammatory phenomena and progression of lung damage]
G Borzone1, B Ramírez, T Reyes
1Depto. Enfermedades Respiratorias, Pontificia Universidad Católica de Chile, Santiago, Chile. gborzone@med.puc.cl
Background:
Although the hamster model of elastase induced emphysema is well characterized, the rat model has received less attention.
Aim:
To evaluate the effect of a single intratracheal elastase dose on lung pathological changes of Sprague-Dawley rats.
Material And Methods:
Rats were injected with a single intratracheal elastase dose of 28 U/100 g body weight or saline and studied 7, 15, 30 and 365 days after injection.
Results:
Forty percent of rats died in the first 48 hours after injection, six were sacrificed at 7 days, 6 at 15 days, 7 at 30 days and 12 at 365 days. Progressive centroacinar emphysema was found from day 7 after elastase, with a persistent inflammatory reaction in the vicinity of emphysematous areas.
Conclusions:
Present findings differ from the panacinar emphysema described in the hamster using a similar elastase dose.