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Identification and partial characterization of specific oestrogen-binding components in human kidney
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|November 1, 1976
Summary
Researchers identified specific estrogen receptors in normal adult male human kidney cytosol. These receptors bind 17beta-estradiol with high affinity, suggesting a role for estrogens in kidney function.
Area of Science:
- Endocrinology
- Molecular Biology
- Urology
Background:
- Estrogen receptors are crucial in various physiological processes.
- Their presence and function in the human kidney are not fully elucidated.
Purpose of the Study:
- To identify and characterize cytoplasmic components in normal adult male human kidney that specifically bind 17beta-estradiol.
- To determine the properties and specificity of these binding components.
Main Methods:
- Dextran-coated charcoal assay for binding capacity and affinity.
- Sucrose gradient centrifugation for sedimentation analysis.
- Agar gel electrophoresis for separation and identification of binding entities.
Main Results:
- Specific high-affinity binding sites for 17beta-estradiol were detected in human kidney cytosol.
- The dissociation constant was 2.2 x 10(-9) mol/l, with a binding capacity of 34.0 fmol/mg protein.
- Binding components were primarily in the 4S region and distinct from sex hormone-binding globulin.
- Ligand specificity confirmed a requirement for estrogens, independent of mineralocorticoid receptors.
Conclusions:
- The human kidney contains specific binding components with characteristics of estrogen receptors.
- These findings suggest a potential role for estrogen signaling in the human kidney.