Separation of prostaglandins E2 and F2alpha from their pulmonary metabolites by thin-layer chromatography

A simple thin-layer chromatographic system has been developed separate PGE2 and PGF2alpha from 13, 14-dihydro-PGF2alpha, and other pulmonary metabolites of prostaglandins. The separation is achieved on Kieselgel-60 F-254 plates impregnated with both silver nitrate and boric acid. The system should be of use in the purification and tentative identification of primary prostaglandin.