Related Experiment Videos
A polymerase chain reaction-based screening method for transgenic Arabidopsis
V De Neef1, W Van Caeneghem, M Marichal
1Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, Belgium.
Genetic Analysis : Biomolecular Engineering
|March 20, 1999
Abstract:
A simple and rapid PCR-based method for screening transformed Arabidopsis plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.