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Related Experiment Videos

Strain dependency of TGFbeta1 function during embryogenesis.

S Kallapur1, I Ormsby, T Doetschman

  • 1Division of Neonatology and Pulmonary Biology, Children's Hospital Medical Center, University of Cincinnati, Ohio 45229, USA. kalls0@chmcc.org

Molecular Reproduction and Development
|March 26, 1999
PubMed
Summary
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Transforming growth factor beta 1 (TGFβ1) is crucial for embryonic development, impacting both pre-implantation stages and yolk sac formation. Genetic background significantly influences the lethality observed in TGFβ1 knockout embryos.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Transforming growth factor beta 1 (TGFβ1) plays a role in embryonic development.
  • Incomplete penetrance of TGFβ1 knockout phenotypes complicates understanding its precise functions.
  • Previous studies indicated lethality due to yolk sac defects in mixed genetic backgrounds.

Purpose of the Study:

  • To investigate the role of TGFβ1 in early embryogenesis.
  • To determine the impact of different genetic backgrounds on TGFβ1 knockout lethality.
  • To identify distinct developmental stages affected by the absence of TGFβ1.

Main Methods:

  • Generation of TGFβ1 knockout mouse models (Tgfb1-/- and Tgfb1+/-).
  • Analysis of embryonic lethality across different genetic backgrounds (e.g., CF-1, NIH/Ola x C57BL/6J/Ola x 129).

Related Experiment Videos

  • Assessment of developmental stages, including pre-implantation and yolk sac development.

    • Main Results:

    • On a CF-1 background, TGFβ1 deficiency causes pre-morula lethality in approximately 50% of null embryos.
    • Embryonic lethality in Tgfb1-/- embryos varies from 20% to 100% depending on the genetic background.
    • Pre-implantation lethality susceptibility is influenced by genetic modifiers, as shown by increased penetrance on a C57BL/6 background.

    Conclusions:

    • TGFβ1 is essential for two critical phases of embryogenesis: pre-implantation development and yolk sac vasculogenesis/hematopoiesis.
    • Genetic background is a major determinant of lethality in TGFβ1 knockout embryos.
    • Targeted genetic modifications can reveal the full spectrum of TGFβ1's essential developmental roles.