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Updated: Jul 5, 2026

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
Published on: March 31, 2011
Multilineage potential of adult human mesenchymal stem cells
M F Pittenger1, A M Mackay, S C Beck
1Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231-3043, USA. mpittenger@osiristx.com
Abstract:
Human mesenchymal stem cells are thought to be multipotent cells, which are present in adult marrow, that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues, including bone, cartilage, fat, tendon, muscle, and marrow stroma. Cells that have the characteristics of human mesenchymal stem cells were isolated from marrow aspirates of volunteer donors. These cells displayed a stable phenotype and remained as a monolayer in vitro. These adult stem cells could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages. Individual stem cells were identified that, when expanded to colonies, retained their multilineage potential.
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Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
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