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Updated: Jul 31, 2026

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis
Published on: August 15, 2013
[The transcription factor STAT3 is associated with elements of the cytoskeleton in A-431 cells]
A L Evdonin1, I P Sokolova, N D Medvedeva
1Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Abstract:
The transcription factor STAT3 (signal transducers and activators of transcription) takes part in cell signaling. Here we show that STAT3 is associated with the cytoskeleton in A-431 cells. The protein is determined in cytoskeletal (detergent insoluble) fraction of control cells and of cells after RGF treatment. STAT3 is diffusely distributed in the cytoplasm of control cells to be seen relocalized to the nuclei after EGF treatment. Besides, the protein is observed in cell membrane protrusions. Using double immunofluorescence, co-localization of action and STAT3 was shown in these structures.
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