Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatially resolved force spectroscopy of biological surfaces using the atomic force microscope.

W F Heinz1, J H Hoh

  • 1Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Trends in Biotechnology
|April 17, 1999
PubMed
Summary

Atomic force microscopy maps intermolecular forces and material properties at the nanoscale. These detailed surface maps offer fundamental insights into biological structures and advanced material characterization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of DNA condensates by atomic force microscopy.

Methods in molecular medicine·2011
Same author

Probing the machinery of intracellular trafficking with the atomic force microscope.

Traffic (Copenhagen, Denmark)·2001
Same author

Predicting properties of intrinsically unstructured proteins.

Progress in biophysics and molecular biology·2001
Same author

AFM force measurements on microtubule-associated proteins: the projection domain exerts a long-range repulsive force.

FEBS letters·2001
Same author

Cationic silanes stabilize intermediates in DNA condensation.

FEBS letters·1999
Same author

Automated sizing of DNA fragments in atomic force microscope images.

Medical & biological engineering & computing·1999

Area of Science:

  • Biophysics
  • Materials Science
  • Nanotechnology

Background:

  • Macromolecular interactions are fundamentally governed by the spatial distribution of intermolecular forces.
  • Understanding these forces is crucial for both biological systems and technological applications.

Purpose of the Study:

  • To highlight the utility of atomic force microscopy (AFM) in mapping nanoscale interaction forces.
  • To demonstrate AFM's capability in characterizing surface and material properties.

Main Methods:

  • Utilizing atomic force microscopy (AFM) to investigate interaction forces between nanometer-scale objects.
  • Generating spatially resolved maps of sample properties, including charge density, adhesion, and stiffness.
  • Measuring the force required to break specific ligand-receptor bonds.

Related Experiment Videos

Main Results:

  • AFM enables the creation of detailed maps of surface properties at the nanoscale.
  • The technique can quantify various interaction forces, such as adhesion and ligand-receptor bond strength.
  • Spatially resolved data provides insights into localized material characteristics.

Conclusions:

  • Spatially resolved force mapping using AFM provides fundamental insights into biological structure.
  • AFM is an important emerging tool for characterizing technologically relevant biological systems.
  • This technique advances the understanding and application of nanoscale interactions.