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Related Experiment Videos

Lectin-based affinity capture for MALDI-MS analysis of bacteria.

J Bundy1, C Fenselau

  • 1Department of Chemistry and Biochemistry, University of Maryland, College Park College Park 20742, USA.

Analytical Chemistry
|April 16, 1999
PubMed
Summary

Immobilized lectins on affinity surfaces enable sample concentration and isolation for mass spectrometry. This method effectively removes contaminants, allowing characterization of carbohydrates and bacterial species.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Surface Chemistry

Background:

  • Mass spectrometry is a powerful analytical technique.
  • Contaminants in samples can interfere with mass spectrometry analysis.
  • Affinity-based isolation methods can improve sample quality.

Purpose of the Study:

  • To develop an affinity surface for sample isolation and concentration.
  • To enable mass spectrometric analysis of complex samples.
  • To remove contaminants detrimental to MALDI mass spectrometry.

Main Methods:

  • Immobilization of lectins (Concanavalin A) onto gold foil using self-assembled monolayers.
  • Application of samples (containing carbohydrates or bacteria) in various solutions (e.g., urine, buffer) to the lectin-derivatized surface.

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  • Washing the surface to remove unbound components and contaminants.
  • Analysis of captured samples using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).

    • Main Results:

    • Successful isolation and concentration of carbohydrates and bacterial species.
    • Effective removal of salts, urea, buffers, and other contaminants.
    • Characterization of samples at relatively low levels using MALDI-TOF MS.
    • Demonstration of the utility of lectin-functionalized surfaces for sample preparation.

    Conclusions:

    • Immobilized lectins provide an effective platform for affinity-based sample enrichment.
    • This approach enhances sample purity for mass spectrometry.
    • The method is applicable to diverse sample types and matrices, including urine.