Issues in immunohistochemistry
Related Experiment Video
Updated: Aug 8, 2026
09:15
Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry
Published on: July 26, 2019
1Pharmacia and Upjohn, Kalamazoo, Michigan, USA. cwjohnso@am.pnu.com
Toxicologic Pathology
|April 20, 1999
Abstract:
Immunohistochemistry is a powerful technique used to localize specific molecules in tissues. Literally thousands of immunoreagents specific for an incredibly diverse number of molecules are commercially available, and some of these immunoreagents will detect minute quantities of target molecule in tissues. However, like any powerful technique, the staining study design must be carefully conceived and controlled to avoid misinterpretation.
Related Concept Videos
01:22Immunocytochemistry and Immunohistochemistry
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
These...
01:20Immunogold Electron Microscopy
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.