Self-gelatinizable copolymer immobilized glucose biosensor based on prussian blue modified graphite electrode

Area of Science:

• Biomedical Engineering
• Electrochemistry
• Polymer Science

Background:

• Development of sensitive and stable glucose biosensors is crucial for diabetes management.
• Immobilization of enzymes like glucose oxidase is key for biosensor performance.
• Prussian Blue (PB) is an effective mediator for electrochemical detection of hydrogen peroxide.

Purpose of the Study:

• To develop a novel amperometric glucose biosensor with enhanced stability and sensitivity.
• To utilize a self-gelatinizable copolymer for enzyme immobilization and electrode modification.
• To evaluate the performance of the biosensor for glucose determination in biological samples.

Main Methods:

• Synthesis of a poly(vinyl alcohol) grafting 4-vinylpyridine copolymer for enzyme immobilization.
• Modification of a graphite electrode with Prussian Blue (PB).
• Amperometric detection of hydrogen peroxide produced by glucose oxidase-catalyzed glucose oxidation.

Main Results:

• The developed biosensor demonstrated high sensitivity and stability.
• The apparent Michaelis-Menten constant was 18 +/- 0.2 mM, with a linear range from 5 microM to 4.5 mM.
• The detection limit was as low as 0.5 microM glucose, and results correlated well with hospital spectroscopy methods for serum samples.

Conclusions:

• The novel copolymer provides excellent biocompatibility and firm adhesion for immobilizing glucose oxidase.
• The PB-modified electrode exhibits efficient catalytic activity for hydrogen peroxide reduction.
• The developed glucose biosensor is a promising tool for accurate glucose monitoring in clinical settings.