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[Streptococcus pneumoniae adhesion to mouse tracheal explants and its inhibition by carbohydrate preparations]
L A Vishniakova1, Iu V Reztsova
1State Research Center of Pulmonology, St. Petersburg, Russia.
Abstract:
The method for the cultivation of tracheal explants of non-inbred mice was developed. The adhesive capacity of 34 S. pneumoniae strains belonging to 15 serotypes was studied. The geometric mean of S. pneumoniae concentrations attached to the organ culture was 8.93 x 10(4) CFU/ml. D-galactose blocked adhesion, on the average, 4.35 x 10(4) (95.5%) CFU/ml. The level of S. pneumoniae adhesion and the degree of its inhibition were dose- and strain-dependent. The use of different variants of the combination of D-galactose and S. pneumoniae made it possible to come to the conclusion that this monosaccharide blocked the receptors of epithelial cells and bacterial adhesins.
Insights
Researchers developed a method to study Streptococcus pneumoniae (S. pneumoniae) adhesion to mouse tracheal explants. D-galactose significantly blocked bacterial attachment, indicating its role in inhibiting S. pneumoniae colonization.
Area of Science:
- Microbiology
- Cell Biology
- Immunology
Context:
- Streptococcus pneumoniae (S. pneumoniae) is a significant human pathogen.
- Understanding bacterial adhesion mechanisms is crucial for developing effective prevention and treatment strategies.
- Tracheal explant models provide an ex vivo system to study host-pathogen interactions in the respiratory tract.
Purpose:
- To develop a method for cultivating mouse tracheal explants for studying bacterial adhesion.
- To investigate the adhesive capacity of various S. pneumoniae strains.
- To determine the role of D-galactose in inhibiting S. pneumoniae adhesion to tracheal epithelial cells.
Summary:
- A novel method for cultivating mouse tracheal explants was established.
- The adhesion of 34 S. pneumoniae strains (15 serotypes) to organ cultures was quantified (geometric mean: 8.93 x 10^4 CFU/ml).
- D-galactose effectively blocked S. pneumoniae adhesion by 95.5% (4.35 x 10^4 CFU/ml), demonstrating dose- and strain-dependent inhibition.
Impact:
- This study elucidates the mechanism by which D-galactose inhibits S. pneumoniae adhesion, likely by targeting epithelial cell receptors and bacterial adhesins.
- The findings contribute to understanding S. pneumoniae pathogenesis and may inform the development of novel anti-adhesion therapies.
- The established tracheal explant model serves as a valuable tool for future respiratory pathogen research.