[Quantitative assay of autoantibodies against low density lipoproteins]

The task of this study was to develop a solid-phase enzyme immunoassay (EIA) for measuring autoantibodies to low-density lipoproteins. The sandwich variant of solid-phase EIA was used. Parameters of the test were selected. Test system was calibrated for high-affinity autoantibodies obtained on an immunoadsorbent from a serum pool of coronary patients with hyperlipidemia. Normal values of autoantibodies in human serum (45.32 +/- 4.25) x 10(-3) g/liter and in circulating immune complexes (0.52 +/- 0.07) x 10(-3) g/liter for this method are determined. In coronary patients with angiographically documented atherosclerosis the level of autoantibodies was significantly higher: (115-175) x 10(-3) g/liter (p < 0.001) in the blood serum and (1.1-5.2) x 10(-3) g/liter (p < 0.01) in circulating immune complexes.