Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

[Headspace solid phase micro-extraction method optimization for residual solvent analysis].

C Camarasu¹, M Mezei, A Szabó

¹Központi Analitikai Laboratórium, Richter Gedeon Rt., Budapest.

Acta Pharmaceutica Hungarica

|July 2, 1999

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of predelivery fetal echocardiography, cardiac magnetic resonance imaging and acute maternal hyperoxygenation in predicting urgency of neonatal balloon atrial septostomy in fetal dextro-transposition of the great arteries.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2026

Same author

Rate of prenatal diagnosis of critical congenital heart disease continued to improve despite COVID-19 pandemic: multicenter Canadian study.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2025

Same author

Hippocampal recording with a soft microelectrode array in a cranial window imaging scheme: a validation study.

Scientific reports·2024

Same author

Solitary humpback whales manufacture bubble-nets as tools to increase prey intake.

Royal Society open science·2024

Same author

Presentation of Meibomian Acini Compared to Dermal Papillae of the Eyelid Margin, Using Confocal Laser Scanning Microscopy and Corresponding Histology.

Klinische Monatsblatter fur Augenheilkunde·2024

Same author

Safety and feasibility pilot study of continuous low-dose maternal supplemental oxygen in fetal single ventricle heart disease.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2024

Optimized headspace SPME for residual solvent analysis using a PDMS/DVB fiber achieved sensitive and precise results. Key factors include equilibration time, headspace volume, organic content, and salt addition for robust analysis.

Area of Science:

Analytical Chemistry
Chromatography
Method Development

Context:

Residual solvent analysis is crucial for pharmaceutical and chemical industries.
Headspace Solid-Phase Microextraction (SPME) offers a solvent-free approach for volatile compound analysis.
Optimization of SPME parameters is essential for achieving reliable and sensitive results.

Purpose:

To systematically optimize headspace SPME conditions for residual solvent analysis.
To evaluate the performance of a polydimethyl-siloxane/divinylbenzene (PDMS/DVB) fiber.
To establish optimal parameters for chromatographic system, extraction, and desorption.

Summary:

Optimized chromatographic conditions included a narrow bore capillary column, low starting temperature, and a narrow injector liner.

Related Experiment Videos

Optimal extraction parameters were identified as 30 min equilibration time, 4.6 ml headspace volume, 0.1-1% total organic content, and 2 g NaCl addition.

Optimal desorption temperature was 220°C, with the PDMS/DVB fiber demonstrating superior detection limits (10-100 pg/ml) and precision (1-3% RSD).

Impact:

Provides a validated and optimized headspace SPME method for accurate residual solvent determination.
The optimized method offers improved sensitivity and precision compared to previous techniques.
Facilitates robust quality control in industries requiring residual solvent analysis.